CCL27 ELISA 试剂盒
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- 抗原 See all CCL27 ELISA试剂盒
- CCL27 (Chemokine (C-C Motif) Ligand 27 (CCL27))
- 抗原表位
- AA 25-112
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL27/CTACK
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: F25-G112 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: F25-G112 - Featured
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Chemokine (C-C Motif) Ligand 27 (CCL27) ELISA Kit
CCL27 适用: 人 Colorimetric Sandwich ELISA 0.313-20 ng/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 27 (CCL27) ELISA KitCCL27 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 27 (CCL27) ELISA KitCCL27 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 27 (CCL27) ELISA KitCCL27 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 27 (CCL27) ELISA KitCCL27 适用: 小鼠 AA 26-120 Colorimetric Sandwich ELISA
Chemokine (C-C Motif) Ligand 27 (CCL27) ELISA KitCCL27 适用: 小鼠 Colorimetric Sandwich ELISA 15-18000 pg/mL Cell Culture Supernatant, Plasma, Serum
Chemokine (C-C Motif) Ligand 27 (CCL27) ELISA KitCCL27 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.
Tissue Specificity: Testis, thymus, placenta, ovary and skin.
- 板类型
- Pre-coated
- 实验流程
- human CCL27 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL27 has been precoated onto 96-well plates. Standards(E.coli, F25-G112) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL27 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL27 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CCL27 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CCL27 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 352, Standard deviation: 14.78, CV(%): 4.2
- Sample 2: n=16, Mean(pg/ml): 794, Standard deviation: 30.97, CV(%): 3.9
- Sample 3: n=16, Mean(pg/ml): 1456, Standard deviation: 77.17, CV(%): 5.3,
- Sample 1: n=24, Mean(pg/ml): 376, Standard deviation: 31.58, CV(%): 8.4
- Sample 2: n=24, Mean(pg/ml): 822, Standard deviation: 64.94, CV(%): 7.9
- Sample 3: n=24, Mean(pg/ml): 1570, Standard deviation: 111.47, CV(%): 7.1
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all CCL27 ELISA试剂盒
- CCL27 (Chemokine (C-C Motif) Ligand 27 (CCL27))
- 别名
- CCL27 (CCL27 产品)
- 别名
- ALP ELISA Kit, ILC ELISA Kit, CTACK ELISA Kit, ESkine ELISA Kit, CCL27 ELISA Kit, CTAK ELISA Kit, ESKINE ELISA Kit, PESKY ELISA Kit, SCYA27 ELISA Kit, AW558992 ELISA Kit, Ccl27 ELISA Kit, Scya27 ELISA Kit, Scya27a ELISA Kit, C-C motif chemokine ligand 27 ELISA Kit, uncharacterized LOC508933 ELISA Kit, chemokine (C-C motif) ligand 27A ELISA Kit, Ccl27 ELISA Kit, CCL27 ELISA Kit, LOC508933 ELISA Kit, Ccl27a ELISA Kit
- 背景
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Protein Function: Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.
Background: Chemokine(C-C motif) ligand 27(CCL27) is a small cytokine belonging to the CC chemokine family, it is also known under the names IL-11 R-alpha-locus chemokine(ILC), Skinkine, ESkine and Cutaneous T-cell-attracting chemokine(CTACK). This gene is mapped to 9p13.3. CCL27 is associated with homing of memory T lymphocytes to the skin, and plays a role in T cell-mediatedinflammation of the skin. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin. It elicits its chemotacticeffects by binding to the chemokine receptor CCR10.
Synonyms: C-C motif chemokine 27,CC chemokine ILC,Cutaneous T-cell-attracting chemokine,CTACK,ESkine,IL-11 R-alpha-locus chemokine,Skinkine,Small-inducible cytokine A27,CCL27,ILC, SCYA27,
Full Gene Name: C-C motif chemokine 27
Cellular Localisation: Secreted. - 基因ID
- 10850
- UniProt
- Q9Y4X3
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