(3 references)Our Local Distributor
北京 101111
Quick Overview for SensoLyte® Green Protease Assay Kit (ABIN1882589)
检测方法
应用范围
-
-
产品特性
-
The SensoLyte® Green Protease Assay Kit is widely used for detection of generic protease activities. The kit uses a casein derivative that is heavily labeled with a rhodamine derivative, resulting in almost total quenching of the conjugate's fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity (Ex/Em=488/520 nm). The SensoLyte® protease assay kits do not require any separation steps and can be used to continuously measure the kinetics of a variety of exopeptidases and endopeptidases. The kit contains:
Fluorescent labeled casein with high ratio of dye/protein (pH-insensitive)
Trypsin (as positive control)
Assay buffer
A detailed protocol
-
-
Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lipopolysaccharides (LPS) ELISA Kit适用: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Amyloid beta 1-42 (Abeta 1-42) ELISA KitAbeta 1-42 适用: 人 Colorimetric Sandwich ELISA 4.688 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate
Amyloid beta 1-40 (Abeta 1-40) ELISA KitAbeta 1-40 适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 适用: 小鼠 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha 适用: 人 AA 77-233 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 小鼠 AA 129-247 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
-
-
说明
-
FRET-based Assay Kit
-
限制
- 仅限研究用
-
-
-
注意事项
- Protect Component A from light and moisture.
-
储存条件
- -20 °C
-
储存方法
- Store Components A and B at -20 °C. Component C can be stored at room temperature for convenience.
-
-
-
: "Inhibitory effects of antrodins A-E from Antrodia cinnamomea and their metabolites on hepatitis C virus protease." in: Phytotherapy research : PTR, Vol. 23, Issue 4, pp. 582-4, (2009) (PubMed).
: "Synthesis of dammarane-type triterpene derivatives and their ability to inhibit HIV and HCV proteases." in: Bioorganic & medicinal chemistry, Vol. 17, Issue 8, pp. 3003-10, (2009) (PubMed).
: "A plant pathogen virulence factor inhibits the eukaryotic proteasome by a novel mechanism." in: Nature, Vol. 452, Issue 7188, pp. 755-8, (2008) (PubMed).
-
-
