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SensoLyte® AFC Thrombin Activity Assay Kit

Act, FRET Fluorometric

Activity Assay (AcA) image for SensoLyte® AFC Thrombin Activity Assay Kit (ABIN1882568) (SensoLyte® AFC Thrombin Activity Assay Kit)

1 image

(3 references)

产品编号 ABIN1882568

发货至: 中国

Quick Overview for SensoLyte® AFC Thrombin Activity Assay Kit (ABIN1882568)

抗原

alpha-Thrombin

检测方法

Fluorometric

应用范围

Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)

品牌

SensoLyte®

产品特性

The SensoLyte® AFC Thrombin Assay Kit is optimized for screening of enzyme inhibitors. This kit contains a fluorogenic substrate which shows high reactivity and low background. Thrombin cleaves the substrate resulting in the release of the AFC (7-amido-4-trifluoromethylcoumarin) fluorophore. Fluorescence can be monitored at excitation/emission= 380 nm/500 nm. The assays are performed in a convenient 96-well or 384-well microplate format.
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适用: 人 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum

alpha-Thrombin ELISA Kit
适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum

alpha-Thrombin ELISA Kit
适用: 猴 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate

alpha-Thrombin ELISA Kit
适用: 兔 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate

SensoLyte® 520 Thrombin Activity Assay Kit
Act, FRET Fluorometric

alpha-Thrombin ELISA Kit
适用: 猴 Colorimetric Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

alpha-Thrombin ELISA Kit
适用: 豚鼠 Colorimetric Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

alpha-Thrombin ELISA Kit
适用: 小鸡 Colorimetric Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

alpha-Thrombin ELISA Kit
适用: 山羊 Colorimetric Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

说明

FRET-based Assay Kit

限制

仅限研究用
注意事项

Aliquot as needed to avoid multiple freeze-thaw cycles. Protect Components A and B from light and moisture.

储存条件

-80 °C

储存方法

Store all kit components, except Component D, at -20 °C. Store Component D at -80 °C. Components C and F can be stored at room temperature for convenience.
Wendeler, Pabst, Wang, Strouse, Wang, Hunter: "Process-scale purification and analytical characterization of highly gamma-carboxylated recombinant human prothrombin." in: Journal of chromatography. A, Vol. 1325, pp. 171-8, (2014) (PubMed).

Yang, Ko, Yang, Kim, Seo, Park, Choi, Seong, Kweon: "Interleukin-1 promotes coagulation, which is necessary for protective immunity in the lung against Streptococcus pneumoniae infection." in: The Journal of infectious diseases, Vol. 207, Issue 1, pp. 50-60, (2012) (PubMed).

Lim, Joy, Hill, San Brian Chia: "Novel agmatine and agmatine-like peptidomimetic inhibitors of the West Nile virus NS2B/NS3 serine protease." in: European journal of medicinal chemistry, Vol. 46, Issue 7, pp. 3130-4, (2011) (PubMed).
抗原

alpha-Thrombin

别名

Thrombin

背景

Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation. Thrombin also influences a number of normal and pathological processes, including inflammation, tissue repair, embryogenesis, angiogenesis, and tumor invasion.

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