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Quick Overview for SensoLyte® AFC Thrombin Activity Assay Kit (ABIN1882568)
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检测方法
应用范围
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产品特性
- The SensoLyte® AFC Thrombin Assay Kit is optimized for screening of enzyme inhibitors. This kit contains a fluorogenic substrate which shows high reactivity and low background. Thrombin cleaves the substrate resulting in the release of the AFC (7-amido-4-trifluoromethylcoumarin) fluorophore. Fluorescence can be monitored at excitation/emission= 380 nm/500 nm. The assays are performed in a convenient 96-well or 384-well microplate format.
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alpha-Thrombin ELISA Kit
适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
alpha-Thrombin ELISA Kit适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
alpha-Thrombin ELISA Kit适用: 人 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
alpha-Thrombin ELISA Kit适用: 猴 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
alpha-Thrombin ELISA Kit适用: 兔 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
SensoLyte® 520 Thrombin Activity Assay KitAct, FRET Fluorometric
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说明
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FRET-based Assay Kit
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限制
- 仅限研究用
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注意事项
- Aliquot as needed to avoid multiple freeze-thaw cycles. Protect Components A and B from light and moisture.
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储存条件
- -80 °C
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储存方法
- Store all kit components, except Component D, at -20 °C. Store Component D at -80 °C. Components C and F can be stored at room temperature for convenience.
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: "Process-scale purification and analytical characterization of highly gamma-carboxylated recombinant human prothrombin." in: Journal of chromatography. A, Vol. 1325, pp. 171-8, (2014) (PubMed).
: "Interleukin-1 promotes coagulation, which is necessary for protective immunity in the lung against Streptococcus pneumoniae infection." in: The Journal of infectious diseases, Vol. 207, Issue 1, pp. 50-60, (2012) (PubMed).
: "Novel agmatine and agmatine-like peptidomimetic inhibitors of the West Nile virus NS2B/NS3 serine protease." in: European journal of medicinal chemistry, Vol. 46, Issue 7, pp. 3130-4, (2011) (PubMed).
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: "Process-scale purification and analytical characterization of highly gamma-carboxylated recombinant human prothrombin." in: Journal of chromatography. A, Vol. 1325, pp. 171-8, (2014) (PubMed).
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- alpha-Thrombin
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别名
- Thrombin
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背景
- Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation. Thrombin also influences a number of normal and pathological processes, including inflammation, tissue repair, embryogenesis, angiogenesis, and tumor invasion.
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