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Quick Overview for SensoLyte® 520 Thrombin Activity Assay Kit (ABIN1882567)
抗原
检测方法
应用范围
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产品特性
- The SensoLyte® 520 Thrombin Assay Kit is optimized for detecting thrombin activity. This kit contains a novel internally quenched 5-FAM/QXL™ 520 FRET substrate for thrombin. Thrombin cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence which can be monitored at excitation/emission= 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assay can be performed in a convenient 96-well or 384-well microplate format for screening of enzyme inhibitors.
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alpha-Thrombin ELISA Kit
适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
alpha-Thrombin ELISA Kit适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
alpha-Thrombin ELISA Kit适用: 人 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
alpha-Thrombin ELISA Kit适用: 猴 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
alpha-Thrombin ELISA Kit适用: 兔 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
SensoLyte® AFC Thrombin Activity Assay KitAct, FRET Fluorometric
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说明
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FRET-based Assay Kit
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限制
- 仅限研究用
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注意事项
- Aliquot as needed to avoid multiple freeze-thaw cycles. Protect Components A and B from light and moisture.
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储存条件
- -80 °C
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储存方法
- Store all kit components, except Component D, at -20 °C. Store Component D at -80 °C. Components C and F can be stored at room temperature for convenience.
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: "Ion-responsive hemin-G-quadruplexes for switchable DNAzyme and enzyme functions." in: Chemistry (Weinheim an der Bergstrasse, Germany), Vol. 20, Issue 19, pp. 5619-24, (2014) (PubMed).
: "Effect of thrombin on human amnion mesenchymal cells, mouse fetal membranes, and preterm birth." in: The Journal of biological chemistry, Vol. 289, Issue 19, pp. 13295-307, (2014) (PubMed).
: "System-level multi-target drug discovery from natural products with applications to cardiovascular diseases." in: Molecular diversity, Vol. 18, Issue 3, pp. 621-35, (2014) (PubMed).
: "High thrombin concentrations in fibrin sealants induce apoptosis in human keratinocytes." in: Journal of biomedical materials research. Part A, Vol. 100, Issue 5, pp. 1239-47, (2012) (PubMed).
: "Antimicrobial and anticoagulant activities of N-chlorotaurine, N,N-dichloro-2,2-dimethyltaurine, and N-monochloro-2,2-dimethyltaurine in human blood." in: Antimicrobial agents and chemotherapy, Vol. 56, Issue 4, pp. 1979-84, (2012) (PubMed).
: "Immobilization of anticoagulant-loaded liposomes on cell surfaces by DNA hybridization." in: Biomaterials, Vol. 32, Issue 31, pp. 7971-7, (2011) (PubMed).
: "Layer-by-layer co-immobilization of soluble complement receptor 1 and heparin on islets." in: Biomaterials, Vol. 32, Issue 27, pp. 6487-92, (2011) (PubMed).
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: "Ion-responsive hemin-G-quadruplexes for switchable DNAzyme and enzyme functions." in: Chemistry (Weinheim an der Bergstrasse, Germany), Vol. 20, Issue 19, pp. 5619-24, (2014) (PubMed).
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- alpha-Thrombin
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别名
- Thrombin
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背景
- Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation. Thrombin also influences a number of normal and pathological processes, including inflammation, tissue repair, embryogenesis, angiogenesis, and tumor invasion.
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