(1 reference)Quick Overview for SensoLyte® Green Sirt1 Assay Kit (ABIN1882554)
抗原
See all SIRT1 试剂盒检测方法
应用范围
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品牌
- SensoLyte®
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产品特性
- The SensoLyte® Green SIRT1 Assay Kit provides a convenient, two-step homogeneous procedure for measuring sirtuin 1 activity using a fluorogenic substrate. In the first step, an acetylated substrate is incubated with SIRT1 containing samples. Deacetylation of substrate first sensitizes it, so that, in the second step, mixing with the SIRT developer generates a fluorophore, which can then be detected at Ex/Em= 490 nm/520 nm. This kit can be used for high throughput screening of SIRT1 inhibitors/activators. The long wavelength fluorescence of SIRT1 substrate is less interfered by the autofluorescence of cell components and test compounds. Assays are performed in a standard 96-well format.
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Sirtuin 1 (SIRT1) ELISA Kit
SIRT1 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Sirtuin 1 (SIRT1) ELISA KitSIRT1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Sirtuin 1 (SIRT1) ELISA KitSIRT1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Sirtuin 1 (SIRT1) ELISA KitSIRT1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Sirtuin 1 (SIRT1) ELISA KitSIRT1 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Sirtuin 1 (SIRT1) ELISA KitSIRT1 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
SensoLyte® 520 FRET Sirt1 Assay KitFRET Fluorometric
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说明
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FRET-based Assay Kit
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限制
- 仅限研究用
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注意事项
- Aliquot as needed to avoid freeze-thaw cycles. Protect Components A and B from light and from moisture.
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储存条件
- -80 °C
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储存方法
- Store all kit components, except Component C, at -20 °C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience.
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: "Radioprotective and antioxidant effect of resveratrol in hippocampus by activating Sirt1." in: International journal of molecular sciences, Vol. 15, Issue 4, pp. 5928-39, (2014) (PubMed).
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: "Radioprotective and antioxidant effect of resveratrol in hippocampus by activating Sirt1." in: International journal of molecular sciences, Vol. 15, Issue 4, pp. 5928-39, (2014) (PubMed).
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- SIRT1 (Sirtuin 1 (SIRT1))
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别名
- Sirt1
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背景
- Histone deacetylase (HDAC) enzymes act as transcriptional repressors of genes through the deacetylation of lysine residues on histone proteins. Sirtuins (SIRTs) are a unique class of deacetylases (class III HDACs) that require NAD as a cofactor. Sirtuin 1 (SIRT1) is involved in regulation of tumor suppressor p53 and inhibition of apoptosis. It also represents a target for treatment of age-related diseases and type II diabetes.
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