Quick Overview for SensoLyte® 520 Neprilysin Activity Assay Kit (ABIN1882538)
抗原
See all MME 试剂盒检测方法
应用范围
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品牌
- SensoLyte®
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产品特性
- The SensoLyte® 520 Neprilysin Assay Kit employs a novel internally quenched 5-FAM/QXL™ FRET substrate for the detection of neprilysin activity. The enzyme cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence, which can be monitored at excitation /emission= 490/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assay can detect as low as 0.78 ng/mL of active neprilysin.
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说明
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FRET-based Assay Kit
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限制
- 仅限研究用
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注意事项
- Protect Components A abd B from light and moisture.
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储存条件
- -80 °C
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储存方法
- Store all kit Components at -20 °C, except Component C, which should be stored at -80 °C.
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- MME (Membrane Metallo-Endopeptidase (MME))
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别名
- Neprilysin
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物质类
- Chemical
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背景
- Neprilysin (NEP) is a transmembrane metallopeptidase normally expressed by a variety of tissues. It is also known as neutral endopeptidase, and enkephalinase. NEP cleaves peptides at the N-terminal side of hydrophobic amino acid residues and is responsible for the degradation and inactivation of a variety of physiological substrates. NEP is a major extracellular amyloid beta-peptide degrading enzyme in the brain and targeting NEP is considered a potential therapeutic strategy for the prevention and treatment of Alzheimer's disease. NEP has also been implicated in the pathogenesis of hypertension, diabetes, and cancer.
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