Quick Overview for SensoLyte® 520 MMP-8 Assay Kit (ABIN1882526)
抗原
See all MMP8 试剂盒检测方法
应用范围
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品牌
- SensoLyte®
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产品特性
- SensoLyte® 520 MMP-8 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-8, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
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说明
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FRET-based Assay Kit
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限制
- 仅限研究用
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注意事项
- Protect Components A and B from light.
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储存条件
- -20 °C
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储存方法
- Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.
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: "Neisseria meningitidis induces brain microvascular endothelial cell detachment from the matrix and cleavage of occludin: a role for MMP-8." in: PLoS pathogens, Vol. 6, Issue 4, pp. e1000874, (2010) (PubMed).
: "Real-time impedance analysis of host cell response to meningococcal infection." in: Journal of microbiological methods, Vol. 84, Issue 1, pp. 101-8, (2010) (PubMed).
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: "Neisseria meningitidis induces brain microvascular endothelial cell detachment from the matrix and cleavage of occludin: a role for MMP-8." in: PLoS pathogens, Vol. 6, Issue 4, pp. e1000874, (2010) (PubMed).
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- MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
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别名
- MMP-8
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背景
- Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known. MMP-8 (netrophil collagnease) is proposed as a potential anti-cancer drug target.
抗原
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