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Quick Overview for SensoLyte® 520 MMP-13 Assay Kit (ABIN1882516)
抗原
See all MMP13 试剂盒检测方法
应用范围
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产品特性
- SensoLyte® 520 MMP-13 Assay Kit uses a 5-FAM (fluorophore) and QXL™ 520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon the cleavage of the FRET peptide by MMP-13, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL™ 520 based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
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SensoLyte® Plus 520 MMP-13 Assay Kit Fluorimetric and Enhanced Selectivity
FRET 适用: 人 Fluorometric
SensoLyte® 490 MMP-13 Assay KitFRET Fluorometric
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 兔 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 人 AA 20-471 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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说明
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FRET-based Assay Kit
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限制
- 仅限研究用
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注意事项
- Protect Components A and B from light.
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储存条件
- -20 °C
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储存方法
- Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.
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: "MMP13 is a critical target gene during the progression of osteoarthritis." in: Arthritis research & therapy, Vol. 15, Issue 1, pp. R5, (2014) (PubMed).
: "Urine matrix metalloproteinases (MMPs) as biomarkers for the progression of fracture healing." in: Injury, Vol. 43, Issue 3, pp. 274-8, (2012) (PubMed).
: "Doxycycline-mediated inhibition of matrix metalloproteinases improves healing after rotator cuff repair." in: The American journal of sports medicine, Vol. 38, Issue 2, pp. 308-17, (2010) (PubMed).
: "Matrix metalloproteinase 12-deficiency augments extracellular matrix degrading metalloproteinases and attenuates IL-13-dependent fibrosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 7, pp. 3955-63, (2010) (PubMed).
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: "MMP13 is a critical target gene during the progression of osteoarthritis." in: Arthritis research & therapy, Vol. 15, Issue 1, pp. R5, (2014) (PubMed).
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- MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))
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别名
- MMP-13
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背景
- Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMP-13 (collagenase-3, rat collagenase) is involved in quite a few diseases such as arthritis and asthma.
抗原
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