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Quick Overview for SensoLyte® 520 MMP-14 Assay Kit (ABIN1882505)
抗原
See all MMP14 试剂盒检测方法
应用范围
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产品特性
- SensoLyte® 520 MMP-14 Assay Kit uses a 5-FAM (fluorophore) and QXL™520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon the cleavage of the FRET peptide by MMP-14, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL™520 based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
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Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) ELISA Kit
MMP14 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) ELISA KitMMP14 适用: 人 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) ELISA KitMMP14 适用: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) ELISA KitMMP14 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) ELISA KitMMP14 适用: 大鼠 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
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说明
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FRET-based Assay Kit
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限制
- 仅限研究用
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注意事项
- Protect Components A and B from light.
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储存条件
- -20 °C
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储存方法
- Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.
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: "Graded activation of the MEK1/MT1-MMP axis determines renal epithelial cell tumor phenotype." in: Carcinogenesis, Vol. 32, Issue 12, pp. 1806-14, (2011) (PubMed).
: "LOX-1-MT1-MMP axis is crucial for RhoA and Rac1 activation induced by oxidized low-density lipoprotein in endothelial cells." in: Cardiovascular research, Vol. 84, Issue 1, pp. 127-36, (2009) (PubMed).
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: "Graded activation of the MEK1/MT1-MMP axis determines renal epithelial cell tumor phenotype." in: Carcinogenesis, Vol. 32, Issue 12, pp. 1806-14, (2011) (PubMed).
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- MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))
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别名
- MMP - 14
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背景
- Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. MMP-14 (MT1-MMP) is the membrane bound MMP. When expressed in cells, the catalytic and the hemopexin domains of MMP-14 become exposed on cell surfaces. This enzyme plays an important role in tumor invasion.
抗原
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