SensoLyte® 520 HIV Protease Assay Kit
FRET
适用: Human Immunodeficiency Virus (HIV)
Fluorometric
(2 references)Quick Overview for SensoLyte® 520 HIV Protease Assay Kit (ABIN1882481)
抗原
HIV
(Human Immunodeficiency Virus (HIV))
适用
Human Immunodeficiency Virus (HIV)
检测方法
Fluorometric
应用范围
Fluorescence Resonance Energy Transfer Microscopy (FRET)
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品牌
- SensoLyte®
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产品特性
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The SensoLyte® 520 HIV Assay Kit uses a new FRET peptide substrate that incorporates HiLyte Fluor™ 488 (fluorophore) and QXL™ 520 (quencher) for continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of HiLyte Fluor™ 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by HIV protease, the fluorescence of HiLyte Fluor™ 488 is recovered and can be continuously monitored at:
Excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this HiLyte Fluor™ 488/QXL™ 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components, thus providing better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.
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说明
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FRET-based Assay Kit
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限制
- 仅限研究用
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注意事项
- Protect Components A and B from light.
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储存条件
- -20 °C
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: "Screening of HIV-1 Protease Using a Combination of an Ultra-High-Throughput Fluorescent-Based Assay and RapidFire Mass Spectrometry." in: Journal of biomolecular screening, Vol. 20, Issue 5, pp. 606-15, (2015) (PubMed).
: "Design, asymmetric synthesis, and evaluation of pseudosymmetric sulfoximine inhibitors against HIV-1 protease." in: Bioorganic & medicinal chemistry, Vol. 18, Issue 5, pp. 2037-48, (2010) (PubMed).
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: "Screening of HIV-1 Protease Using a Combination of an Ultra-High-Throughput Fluorescent-Based Assay and RapidFire Mass Spectrometry." in: Journal of biomolecular screening, Vol. 20, Issue 5, pp. 606-15, (2015) (PubMed).
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- HIV (Human Immunodeficiency Virus (HIV))
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物质类
- Virus, Virus
抗原
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