SensoLyte® 490 HCV Protease Assay Kit
FRET
适用: Hepatitis C Virus (HCV)
Fluorometric
1 image
(4 references)Quick Overview for SensoLyte® 490 HCV Protease Assay Kit (ABIN1882472)
抗原
Hepatitis C Virus (HCV)
适用
Hepatitis C Virus (HCV)
检测方法
Fluorometric
应用范围
Fluorescence Resonance Energy Transfer Microscopy (FRET)
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品牌
- SensoLyte®
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产品特性
- The SensoLyte® 490 HCV Protease Assay Kit uses an optimized FRET peptide substrate for the continuous measurement of HCV NS3/4a activity. This FRET substrate is cleaved specifically by HCV NS3/4a protease thereby liberating the C-terminal peptide-fluorophore fragment from the proximity quenching effect of the dark quencher, resulting in a more than 10-fold increase in fluorescence. This assay can detect HCV NS3/4a protease activity at 1 pmole.
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说明
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FRET-based Assay Kit
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限制
- 仅限研究用
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注意事项
- Protect Components A and B from light.
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储存条件
- -20 °C
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储存方法
- Store all kit components at -20 °C. Components C and D can be stored at room temperature for convenience.
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: "2-Arylmethylaminomethyl-5,6-dihydroxychromone derivatives with selective anti-HCV activity." in: Bioorganic & medicinal chemistry letters, Vol. 21, Issue 11, pp. 3202-5, (2011) (PubMed).
: "Ceestatin, a novel small molecule inhibitor of hepatitis C virus replication, inhibits 3-hydroxy-3-methylglutaryl-coenzyme A synthase." in: The Journal of infectious diseases, Vol. 204, Issue 4, pp. 609-16, (2011) (PubMed).
: "Mutations in HCV non-structural genes do not contribute to resistance to nitazoxanide in replicon-containing cells." in: Antiviral research, Vol. 91, Issue 3, pp. 233-40, (2011) (PubMed).
: "A method to simultaneously monitor hepatitis C virus NS3 helicase and protease activities." in: Methods in molecular biology (Clifton, N.J.), Vol. 587, pp. 223-33, (2010) (PubMed).
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: "2-Arylmethylaminomethyl-5,6-dihydroxychromone derivatives with selective anti-HCV activity." in: Bioorganic & medicinal chemistry letters, Vol. 21, Issue 11, pp. 3202-5, (2011) (PubMed).
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- Hepatitis C Virus (HCV)
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物质类
- Virus
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背景
- HCV protease is identified as an important drug-screening target.
抗原
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