SensoLyte® Green Elastase Assay Kit
Quick Overview for SensoLyte® Green Elastase Assay Kit (ABIN1882454)
抗原
See all Elastase 试剂盒检测方法
应用范围
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品牌
- SensoLyte®
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产品特性
- The SensoLyte® Green Elastase Assay Kit uses natural substrate elastin labeled with the 5-FAM fluorophore and the QXL™ 520 quencher. Proteolytic cleavage of labeled elastin yields brightly green fluorescence, which can be continuously monitored at excitation/emission= 488 nm/520 nm. Increase in fluorescence intensity is directly proportional to elastase activity. This kit does not require any separation steps and can be used to continuously measure the kinetics of elastase activity.
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说明
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FRET-based Assay Kit
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限制
- 仅限研究用
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注意事项
- Protect Component A from light and moisture.
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储存条件
- -20 °C
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储存方法
- Store all kit components at -20 °C. Component C can be stored at room temperature for convenience.
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- Elastase
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背景
- Elastases catalyze the hydrolysis of elastin, the chief constituent of elastic fibers that together with collagen determines the mechanical properties of connective tissue. Elastases have many other natural substrates these include proteoglycans, collagens and fibronectin. Implicated in many diseases such as pulmonary emphysema, cystic fibrosis, infections, inflammation and atherosclerosis, elastases are considered important drug targets.
抗原
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