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SensoLyte® 520 Cathepsin K Assay Kit

FRET Fluorometric
产品编号 ABIN1882445
发货至: 中国

Quick Overview for SensoLyte® 520 Cathepsin K Assay Kit (ABIN1882445)

抗原

See all Cathepsin K (CTSK) 试剂盒
Cathepsin K (CTSK)

检测方法

Fluorometric

应用范围

Fluorescence Resonance Energy Transfer Microscopy (FRET)
  • 品牌

    SensoLyte®

    产品特性

    The SensoLyte® 520 Cathepsin K Activity Assay Kit uses a QXL™ 520/HiLyte Fluor™ 488 FRET peptide substrate for the measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte Fluor™ 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490/520 nm. The fluorescent signal from HiLyte Fluor™ 488 is stable at low pH, which is optimal for cathepsin activity. The kit can be used to detect the activity of Cathepsin K enzyme in biological samples and purified enzyme preparations.
  • 说明

    FRET-based Assay Kit

    限制

    仅限研究用
  • 注意事项

    Protect Components A and B from light and moisture.

    储存条件

    -80 °C

    储存方法

    Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience
  • 抗原

    Cathepsin K (CTSK)

    别名

    Cathepsin K

    背景

    Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin K is the lysosomal cysteine protease involved in bone remodeling and resorption. It has potential as a drug target in autoimmune diseases and osteoporosis.
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