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Quick Overview for SensoLyte® 520 Cathepsin G Assay Kit (ABIN1882442)
抗原
See all Cathepsin G (CTSG) 试剂盒检测方法
应用范围
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产品特性
- The SensoLyte® 520 Cathepsin G Activity Assay Kit uses a HiLyte Fluor™ 488/QXL™ 520 FRET peptide substrate for the measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte Fluor™ 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490/520 nm. With superior fluorescence quantum yield and longer emission wavelength, the HiLyte Fluor™ 488/QXL™ 520 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The fluorescent signal from HiLyte Fluor™ 488 is stable at low pH, which is the optimal pH for cathepsin activity. The kit can be used to detect the activity of Cathepsin G enzyme in biological samples and purified enzyme preparations.
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Cathepsin G (CTSG) ELISA Kit
CTSG 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathepsin G (CTSG) ELISA KitCTSG 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cathepsin G (CTSG) ELISA KitCTSG 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Cathepsin G (CTSG) ELISA KitCTSG 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
Cathepsin G (CTSG) ELISA KitHigh Sensitivity CTSG 适用: 小鼠 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Cathepsin G (CTSG) ELISA KitCTSG 适用: 人 Colorimetric Sandwich ELISA 3.125-100 ng/mL Cell Culture Cells, Plasma, Saliva, Serum
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说明
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FRET-based Assay Kit
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限制
- 仅限研究用
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注意事项
- Protect Components A and B from light and moisture.
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储存条件
- -80 °C
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储存方法
- Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience
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: "Serine protease inhibitor A3 in atherosclerosis and aneurysm disease." in: International journal of molecular medicine, Vol. 30, Issue 2, pp. 288-94, (2012) (PubMed).
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: "Serine protease inhibitor A3 in atherosclerosis and aneurysm disease." in: International journal of molecular medicine, Vol. 30, Issue 2, pp. 288-94, (2012) (PubMed).
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- Cathepsin G (CTSG)
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别名
- Cathepsin G
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背景
- Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin G is the serine protease released by neutrophils upon their activation. Cathepsin G is currently being explored as a target for anti-inflammatory and anti-infective drugs.
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