Quick Overview for SensoLyte® 520 Cathepsin E Assay Kit (ABIN1882441)
抗原
See all Cathepsin E (CTSE) 试剂盒检测方法
应用范围
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品牌
- SensoLyte®
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产品特性
- The SensoLyte® 520 Cathepsin E Activity Assay Kit is a homogeneous assay that can be used to detect the enzyme activity in biological samples or in purified enzyme preparations. The unique long wavelength FRET substrate employed in the assay was designed to reduce the cross reactivity of Cathepsin D. A QXL™ 520/HiLyte Fluor™ 488 pair is used for optimal quenching of the intact substrate. When active Cathepsin E cleaves the FRET substrate, it results in an increase of HiLyte Fluor™ 488 fluorescence, monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte Fluor™ 488 is stable at low pH, an optimal pH for cathepsin activity. This assay can detect as low as 2 ng/mL active Cathepsin E.
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Cathepsin E (CTSE) ELISA Kit
CTSE 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cathepsin E (CTSE) ELISA KitCTSE 适用: 人 AA 18-396 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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说明
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FRET-based Assay Kit
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限制
- 仅限研究用
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注意事项
- Protect Components A and B from light and moisture.
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储存条件
- -80 °C
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储存方法
- Store all kit components at -20 °C, except Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience.
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- Cathepsin E (CTSE)
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别名
- Cathepsin E
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背景
- Cathepsin E is a non-lysosomal aspartic proteinase of the pepsin superfamily. It plays an important role in the protein degradation, the generation of bioactive proteins, and antigen processing. Recent studies have particularly suggested that Cathepsin E is important in host defense against cancer cells and invading microorganisms.
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