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SensoLyte® 520 Cathepsin D Assay Kit

FRET Fluorometric
产品编号 ABIN1882439
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for SensoLyte® 520 Cathepsin D Assay Kit (ABIN1882439)

抗原

See all Cathepsin D (CTSD) 试剂盒
Cathepsin D (CTSD)

检测方法

Fluorometric

应用范围

Fluorescence Resonance Energy Transfer Microscopy (FRET)
  • 产品特性

    The SensoLyte® 520 Cathepsin D Activity Assay Kit uses a QXL™ 520/HiLyte Fluor™ 488 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte Fluor™ 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte Fluor™ 488 is stable at low pH, which is optimal for cathepsin activity. The kit can be used to detect the activity of Cathepsin D enzyme in biological samples and purified enzyme preparations.
  • 说明

    FRET-based Assay Kit

    限制

    仅限研究用
  • 注意事项

    Protect Components A and B from light and moisture.

    储存条件

    -80 °C

    储存方法

    Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience
  • Feng, Tang, Kwon, Zong, Hawkins, Kitsis, Pessin: "High-fat diet-induced adipocyte cell death occurs through a cyclophilin D intrinsic signaling pathway independent of adipose tissue inflammation." in: Diabetes, Vol. 60, Issue 8, pp. 2134-43, (2011) (PubMed).

    Li, Lu, Nguyen, Bae, Chapman, Feng, Hawkins, Pessin, Sears, Nguyen, Amidi, Watkins, Nguyen, Olefsky: "Functional heterogeneity of CD11c-positive adipose tissue macrophages in diet-induced obese mice." in: The Journal of biological chemistry, Vol. 285, Issue 20, pp. 15333-45, (2010) (PubMed).

  • 抗原

    Cathepsin D (CTSD)

    别名

    Cathepsin D

    背景

    Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown. Cathepsin D is involved in the pathogenesis of several diseases such as breast cancer and Alzheimer disease.
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