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SensoLyte® 390 Cathepsin D Assay Kit

FRET Fluorometric
产品编号 ABIN1882438
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for SensoLyte® 390 Cathepsin D Assay Kit (ABIN1882438)

抗原

See all Cathepsin D (CTSD) 试剂盒
Cathepsin D (CTSD)

检测方法

Fluorometric

应用范围

Fluorescence Resonance Energy Transfer Microscopy (FRET)
  • 产品特性

    The SensoLyte® 390 Cathepsin D Activity Assay Kit uses a Mca/Dnp FRET peptide for measurement of enzyme activity. In the FRET peptide, the fluorescence of Mca is quenched by Dnp. Upon cleavage into two separate fragments by Cathepsin D, the fluorescence of Mca is recovered, and can be monitored at excitation/emission = 330 nm/390 nm.
  • 说明

    FRET-based Assay Kit

    限制

    仅限研究用
  • 注意事项

    Protect Components A and B from light and moisture.

    储存条件

    -80 °C

    储存方法

    Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience
  • Poliakov, Strunnikova, Jiang, Martinez, Parikh, Lakkaraju, Thomas, Brooks, Redmond: "Multiple A2E treatments lead to melanization of rod outer segment-challenged ARPE-19 cells." in: Molecular vision, Vol. 20, pp. 285-300, (2014) (PubMed).

  • 抗原

    Cathepsin D (CTSD)

    别名

    Cathepsin D

    背景

    Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown. It is involved in the pathogenesis of several diseases such as breast cancer and Alzheimer disease.
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