Quick Overview for SensoLyte® 520 Cathepsin B Assay Kit (ABIN1882436)
抗原
See all Cathepsin B (CTSB) 试剂盒检测方法
应用范围
-
-
品牌
- SensoLyte®
-
产品特性
- The SensoLyte® 520 Cathepsin B Activity Assay Kit provides a QXL™ 520/HiLyte FluorTM 488 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte FluorTM 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, the QXL™ 520/HiLyte FluorTM 488 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
-
-
-
-
说明
-
FRET-based Assay Kit
-
限制
- 仅限研究用
-
-
-
注意事项
- Protect Components A and B from light and moisture.
-
储存条件
- -80 °C
-
储存方法
- Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience
-
-
-
Development of Purkinje cell degeneration in a knockin mouse model reveals lysosomal involvement in the pathogenesis of SCA6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 43, pp. 17693-8, (2012) (PubMed).
: "
-
Development of Purkinje cell degeneration in a knockin mouse model reveals lysosomal involvement in the pathogenesis of SCA6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 43, pp. 17693-8, (2012) (PubMed).
-
- Cathepsin B (CTSB)
-
别名
- Cathepsin B
-
背景
- Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin B is a member of the cathepsin family consisting of 12 cysteine proteases with broad exo- and endopeptidase activity. Elevated levels of cathepsin B were detected in metastases and neurological disorders including Alzheimer's disease (AD).
抗原
-