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Quick Overview for SensoLyte® 520 Cathepsin B Assay Kit (ABIN1882436)
抗原
See all Cathepsin B (CTSB) 试剂盒检测方法
应用范围
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产品特性
- The SensoLyte® 520 Cathepsin B Activity Assay Kit provides a QXL™ 520/HiLyte FluorTM 488 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte FluorTM 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, the QXL™ 520/HiLyte FluorTM 488 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
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Cathepsin B (CTSB) ELISA Kit
CTSB 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Cathepsin B (CTSB) ELISA KitCTSB 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathepsin B (CTSB) ELISA KitCTSB 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Cathepsin B (CTSB) ELISA KitCTSB 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Cathepsin B (CTSB) ELISA KitCTSB 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
SensoLyte® 440 Cathepsin B Assay KitFRET Fluorometric
Cathepsin B (CTSB) ELISA KitCTSB 适用: 小鼠 AA 18-339 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Cathepsin B (CTSB) ELISA KitCTSB 适用: 人 AA 18-339 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Cathepsin B (CTSB) ELISA KitCTSB 适用: 人 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma, Serum
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说明
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FRET-based Assay Kit
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限制
- 仅限研究用
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注意事项
- Protect Components A and B from light and moisture.
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储存条件
- -80 °C
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储存方法
- Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience
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: "Development of Purkinje cell degeneration in a knockin mouse model reveals lysosomal involvement in the pathogenesis of SCA6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 43, pp. 17693-8, (2012) (PubMed).
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: "Development of Purkinje cell degeneration in a knockin mouse model reveals lysosomal involvement in the pathogenesis of SCA6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 43, pp. 17693-8, (2012) (PubMed).
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- Cathepsin B (CTSB)
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别名
- Cathepsin B
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背景
- Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin B is a member of the cathepsin family consisting of 12 cysteine proteases with broad exo- and endopeptidase activity. Elevated levels of cathepsin B were detected in metastases and neurological disorders including Alzheimer's disease (AD).
抗原
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