SensoLyte® Biotin Quantitation Kit
FRET
Fluorometric
产品编号 ABIN1882421
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for SensoLyte® Biotin Quantitation Kit (ABIN1882421)
抗原
Biotin
检测方法
Fluorometric
应用范围
Fluorescence Resonance Energy Transfer Microscopy (FRET)
-
-
标记
- Biotin
-
产品特性
- The SensoLyte® Biotin Quantitation Assay Kit provides a convenient assay for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantifying biotin concentration in a solution. The assay is based on FRET principle. In the first step, avidin, Reagent A tagged with a quencher dye is briefly incubated with biotin samples/standards. In the second step, fluorescent biotin, Reagent B, is added to the assay mixture. The fluorescent signal is directly proportional to the amount of biotin in the sample and can be detected at Ex/Em=490/520 nm. The kit is best used to determine biotin concentration in the range from 2 to 20 picomoles. The kit also provides biotinylated IgG for use as a positive control and a protease for protein digestion to expose hidden biotin groups.
-
-
Biotin ELISA Kit
适用: Various Species Colorimetric Competition ELISA 32 pg/mL - 20000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
-
说明
-
FRET-based Assay Kit
-
限制
- 仅限研究用
-
-
-
注意事项
- Protect Components A and B from light and from moisture.
-
储存条件
- -20 °C
-
储存方法
- Store all kit components at -20 °C. Components E can be stored at room temperature for convenience.
-
-
- Biotin
-
物质类
- Chemical
抗原
-
You are here:
