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Quick Overview for SensoLyte® 520 beta-Secretase (BACE1) Activity Assay Kit (ABIN1882408)
抗原
See all BACE1 试剂盒检测方法
应用范围
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产品特性
- The SensoLyte® 520 beta-Secretase Assay Kit is a homogeneous assay that can be used to detect the activity of enzyme and for screening of secretase inhibitors. It contains a QXL® 520/ HiLyte Fluor™ 488 FRET substrate. The sequence of this FRET peptide is derived from the beta-secretase cleavage site on the Swedish APP mutation. This mutation causes the enhancement of beta-secretase to process APP, resulting in an early onset of AD. Active beta-secretase cleaves FRET substrate into two separate fragments resulting in the release of HiLyte Fluor™ 488 fluorescence that can be monitored at excitation/emission= 490/520 nm. The long wavelength fluorescence of HiLyte Fluor™ 488 is less interfered by the autofluorescence of components in biological samples and test compounds.
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Beta-secretase 1 (BACE1) ELISA Kit
BACE1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Beta-secretase 1 (BACE1) ELISA KitBACE1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Beta-secretase 1 (BACE1) ELISA KitBACE1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Beta-secretase 1 (BACE1) ELISA KitBACE1 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Beta-secretase 1 (BACE1) ELISA KitBACE1 适用: 人 Colorimetric Sandwich ELISA 0.25 ng/mL - 16 ng/mL Cerebrospinal Fluid, Plasma, Serum
Beta-secretase 1 (BACE1) ELISA KitBACE1 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cerebrospinal Fluid, Plasma, Serum
Beta-secretase 1 (BACE1) ELISA KitBACE1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Beta-secretase 1 (BACE1) ELISA KitBACE1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
Beta-secretase 1 (BACE1) ELISA KitBACE1 适用: 人, 小鼠 Colorimetric Sandwich ELISA 1.0-250 ng/mL Cell Culture Supernatant, Plasma, Serum
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说明
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FRET-based Assay Kit
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限制
- 仅限研究用
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储存条件
- -80 °C
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储存方法
- Store all kit components at -20 °C, except for Component F. Store b-secretase (Component F) at -80 °C. Protect Components A and B from light and from moisture. Components D and E can be stored at room temperature for convenience.
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: "Inhibiting ?-secretase activity in Alzheimer's disease cell models with single-chain antibodies specifically targeting APP." in: Journal of molecular biology, Vol. 405, Issue 2, pp. 436-47, (2011) (PubMed).
: "Promotion of BACE1 mRNA alternative splicing reduces amyloid beta-peptide production." in: The Journal of biological chemistry, Vol. 283, Issue 27, pp. 18694-701, (2008) (PubMed).
: "Effect of mycelial extract of Clavicorona pyxidata on the production of amyloid beta-peptide and the inhibition of endogenous beta-secretase activity in vitro." in: Journal of microbiology (Seoul, Korea), Vol. 44, Issue 6, pp. 665-70, (2007) (PubMed).
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: "Inhibiting ?-secretase activity in Alzheimer's disease cell models with single-chain antibodies specifically targeting APP." in: Journal of molecular biology, Vol. 405, Issue 2, pp. 436-47, (2011) (PubMed).
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- BACE1 (Beta-secretase 1 (BACE1))
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别名
- BACE1
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背景
- Beta-Secretase is a membrane-anchored aspartic protease existing in acidic subcellular vesicles. It is a key player in producing beta-amyloid peptides through proteolytic cleavage of the beta-amyloid precursor protein (APP). Accumulation of neurotoxic beta-amyloid peptide is seen in senile plaques in the brains of patients with Alzheimer's disease (AD), an age-related cognitive disorder. Therefore, targeting beta-secretase is considered a potential therapeutic strategy for the prevention and treatment of AD.
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