SensoLyte® 520 ADAM10 Activity Assay Kit
Quick Overview for SensoLyte® 520 ADAM10 Activity Assay Kit (ABIN1882390)
抗原
See all ADAM10 试剂盒检测方法
应用范围
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品牌
- SensoLyte®
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产品特性
- The SensoLyte® 520 ADAM10 Activity Assay Kit is an optimized assay that can be used to detect the ADAM 10 activity. The unique FRET substrate contained in the kit was designed to reduce the cross reactivity with ADAM17 (also called tumor necrosis factor-alpha-converting enzyme, TACE). When active ADAM10 cleaves the FRET substrate, it results in an increase of 5-FAM fluorescence monitored at excitation/emission = 490/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.25 ng/mL active ADAM10.
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说明
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FRET-based Assay Kit
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限制
- 仅限研究用
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注意事项
- Protect components A and B from light and moisture.
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储存条件
- -80 °C
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储存方法
- Store all kit components at -20 °C except component C, which should be stored at -80 °C. Component D can be stored at RT for convenience.
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- ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))
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别名
- ADAM10
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背景
- ADAM10 has been shown to be an effective alpha-secretase in vitro and in vivo. This enzyme acts as a sheddase to cleave cell surface proteins involved in neuropathology, inflammatory response and tumor progression.
抗原
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