NUCB2 ELISA 试剂盒
(2 references)Quick Overview for NUCB2 ELISA 试剂盒 (ABIN1706085)
抗原
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Nesfatin-1
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: V25-L106 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: V25-L106
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Nucleobindin 2 (NUCB2) ELISA Kit
NUCB2 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Nucleobindin 2 (NUCB2) ELISA KitNUCB2 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Nucleobindin 2 (NUCB2) ELISA KitNUCB2 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Nucleobindin 2 (NUCB2) ELISA KitNUCB2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the nucleobindin family.
Tissue Specificity: Predominantly expressed in spleen, testis and normal stomach. .
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板类型
- Pre-coated
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实验流程
- human Nesfatin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Nesfatin-1 has been precoated onto 96-well plates. Standards(E.coli, V25-L106) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Nesfatin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Nesfatin-1 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Nesfatin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Nesfatin-1 standard solution and each sample be measured in duplicate. Seal the plate with the cover and incubate at 37 °C for 90 min.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 146, Standard deviation: 7.6, CV(%): 5.2
- Sample 2: n=16, Mean(pg/ml): 726, Standard deviation: 35.6, CV(%): 4.9
- Sample 3: n=16, Mean(pg/ml): 1252, Standard deviation: 68.86, CV(%): 5.5,
- Sample 1: n=24, Mean(pg/ml): 177, Standard deviation: 9.56, CV(%): 5.4
- Sample 2: n=24, Mean(pg/ml): 838, Standard deviation: 47.8, CV(%): 5.7
- Sample 3: n=24, Mean(pg/ml): 1527, Standard deviation: 96.2, CV(%): 6.3
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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: "Variations in leptin, nesfatin-1 and irisin levels induced by aerobic exercise in young trained and untrained male subjects." in: Biology of sport, Vol. 34, Issue 4, pp. 339-344, (2018) (PubMed).
: "Serum nesfatin-1 and leptin levels in non-obese girls with premature thelarche." in: Journal of endocrinological investigation, Vol. 38, Issue 8, pp. 909-13, (2015) (PubMed).
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: "Variations in leptin, nesfatin-1 and irisin levels induced by aerobic exercise in young trained and untrained male subjects." in: Biology of sport, Vol. 34, Issue 4, pp. 339-344, (2018) (PubMed).
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- NUCB2 (Nucleobindin 2 (NUCB2))
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别名
- NUCB2
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背景
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Protein Function: Calcium-binding protein. May have a role in calcium homeostasis.
Background: Nesfatin-1 is a naturally occurring protein molecule produced by the brains of mammals. It is responsible for regulating appetite and production of body fat. Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the protein precursor, Nucleobindin2(NUCB2). Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues. Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets of Langerhans, gastric endocrine cells and adipocytes. Nesfatin-1 plays a role in hunger and energy regulation in an independent manner.
Synonyms: Nucleobindin-2,DNA-binding protein NEFA,Gastric cancer antigen Zg4,Prepronesfatin,Nesfatin-1,NUCB2,NEFA,
Full Gene Name: Nucleobindin-2
Cellular Localisation: Golgi apparatus . Membrane, Peripheral membrane protein . Cytoplasm . Secreted . Endoplasmic reticulum . Nucleus envelope . Golgi retention is mediated by its N-terminal region. -
基因ID
- 4925
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UniProt
- P80303
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