CCL8 ELISA 试剂盒
Quick Overview for CCL8 ELISA 试剂盒 (ABIN1706084)
抗原
See all CCL8 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CCL8/MCP2
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: E20-P97 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: E20-P97
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Chemokine (C-C Motif) Ligand 8 (CCL8) ELISA Kit
CCL8 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 8 (CCL8) ELISA KitCCL8 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 8 (CCL8) ELISA KitCCL8 适用: 犬 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 8 (CCL8) ELISA KitCCL8 适用: Pig Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Plasma, Serum
Chemokine (C-C Motif) Ligand 8 (CCL8) ELISA KitCCL8 适用: Cow Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 8 (CCL8) ELISA KitCCL8 适用: 马 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 8 (CCL8) ELISA KitCCL8 适用: 人 AA 24-99 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 8 (CCL8) ELISA KitCCL8 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- mouse CCL8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL8 has been precoated onto 96-well plates. Standards(E.coli, E20-P97) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL8 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse CCL8 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse CCL8 standard solution and each sample is measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 32, Standard deviation: 1.31, CV(%): 4.1
- Sample 2: n=16, Mean(pg/ml): 125, Standard deviation: 5.88, CV(%): 4.7
- Sample 3: n=16, Mean(pg/ml): 524, Standard deviation: 27.25, CV(%): 5.2,
- Sample 1: n=24, Mean(pg/ml): 33, Standard deviation: 1.68, CV(%): 5.1
- Sample 2: n=24, Mean(pg/ml): 129, Standard deviation: 8.39, CV(%): 6.5
- Sample 3: n=24, Mean(pg/ml): 531, Standard deviation: 35.58, CV(%): 6.7
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))
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别名
- CCL8
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背景
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Protein Function: Chemotactic factor that attracts monocytes. This protein can bind heparin (By similarity). .
Background: Chemokine(C-C motif) ligand 8, also known as monocyte chemoattractant protein 2(MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. Monocyte chemotactic protein 2(MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.
Synonyms: C-C motif chemokine 8,Monocyte chemoattractant protein 2,Monocyte chemotactic protein 2,MCP-2,Small-inducible cytokine A8,Ccl8,Mcp2, Scya8,
Full Gene Name: C-C motif chemokine 8
Cellular Localisation: Secreted. -
基因ID
- 20307
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UniProt
- Q9Z121
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