Thrombomodulin ELISA 试剂盒
Quick Overview for Thrombomodulin ELISA 试剂盒 (ABIN1706070)
抗原
See all Thrombomodulin (THBD) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Thrombomodulin
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: L17-S517 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: L17-S517
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Thrombomodulin (THBD) ELISA Kit
THBD 适用: 人 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma
Thrombomodulin (THBD) ELISA KitTHBD 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma
Thrombomodulin (THBD) ELISA KitTHBD 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Thrombomodulin (THBD) ELISA KitTHBD 适用: 犬 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma
Thrombomodulin (THBD) ELISA KitTHBD 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma
Thrombomodulin (THBD) ELISA KitTHBD 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Thrombomodulin (THBD) ELISA KitTHBD 适用: 犬 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Thrombomodulin (THBD) ELISA KitTHBD 适用: 大鼠 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Thrombomodulin (THBD) ELISA KitTHBD 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Thrombomodulin (THBD) ELISA KitTHBD 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Contains 1 C-type lectin domain.
Tissue Specificity: Endothelial cells are unique in synthesizing thrombomodulin.
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板类型
- Pre-coated
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实验流程
- mouse Thrombomodulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Thrombomodulin has been precoated onto 96-well plates. Standards(NSO, L17-S517) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Thrombomodulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Thrombomodulin amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse Thrombomodulin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Thrombomodulin standard solution and each sample is measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 257, Standard deviation: 17.5, CV(%): 6.8
- Sample 2: n=16, Mean(pg/ml): 1942, Standard deviation: 101.1, CV(%): 5.2
- Sample 3: n=16, Mean(pg/ml): 3568, Standard deviation: 167.7, CV(%): 4.7,
- Sample 1: n=24, Mean(pg/ml): 366, Standard deviation: 31.8, CV(%): 8.7
- Sample 2: n=24, Mean(pg/ml): 2158, Standard deviation: 153.2, CV(%): 7.1
- Sample 3: n=24, Mean(pg/ml): 3643, Standard deviation: 251.4, CV(%): 6.9
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- Thrombomodulin (THBD)
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别名
- THBD
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背景
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Protein Function: Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
Background: Thrombomodulin(TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. In humans, thrombomodulin is encoded by the THBD gene. The protein has a molecular mass of 74 kDa, and consists of a single chain with 5 distinct domains. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. This raises the speed of protein C activation thousandfold. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor(TAFI,aka carboxypeptidase B2) into its active form. Thrombomodulin is a glycoprotein on the surface of endothelial cells that, in addition to binding thrombin, regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene(THBD) have also been reported to be associated with atypical hemolytic-uremic syndrome(aHUS).
Synonyms: Thrombomodulin,TM,Fetomodulin,CD141,Thbd,
Full Gene Name: Thrombomodulin
Cellular Localisation: Membrane, Single-pass type I membrane protein. -
基因ID
- 21824
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UniProt
- P15306
抗原 See all Thrombomodulin (THBD) ELISA试剂盒
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