alpha 2 Macroglobulin ELISA 试剂盒
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- 抗原 See all alpha 2 Macroglobulin (A2M) ELISA试剂盒
- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 625-40.000 pg/mL
- 最低检测浓度
- 625 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human A2M/alpha2-Macroglobulin
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
- Expression system for standard: from human plasma
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <20pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
- from human plasma
- Featured
- Discover our best selling A2M ELISA Kit
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- Discover our top product A2M ELISA Kit
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alpha-2-Macroglobulin (A2M) ELISA Kit
A2M 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Urine
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 小鼠 Colorimetric Competition ELISA 3.7 ng/mL - 300 ng/mL Plasma, Serum, Urine
alpha-2-Macroglobulin (A2M) ELISA KitHigh Sensitivity A2M 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 大鼠 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Plasma, Serum, Urine
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 人 Colorimetric Competition ELISA 1.25 μg/mL - 20 μg/mL Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 人 Colorimetric Sandwich ELISA 62.5 ng/mL - 4000 ng/mL Cell Culture Supernatant, Plasma, Serum
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 人 Colorimetric Sandwich ELISA 78.125 ng/mL - 5000 ng/mL Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 大鼠 Colorimetric Sandwich ELISA 0.469 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: Cow Colorimetric Sandwich ELISA 0.156 μg/mL - 10 μg/mL Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 兔 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
-
Sequence similarities: Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.
Tissue Specificity: Secreted in plasma. .
- 板类型
- Pre-coated
- 实验流程
- human A2M ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for A2M has been precoated onto 96-well plates. Standards(from human plasma) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for A2M is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human A2M amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 40,000pg/mL, 20,000pg/mL, 10,000pg/mL, 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human A2M standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human A2M standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(ng/ml): 6.18, Standard deviation: 0.377, CV(%): 6.1
- Sample 2: n=16, Mean(ng/ml): 12.04, Standard deviation: 0.771, CV(%): 6.4
- Sample 3: n=16, Mean(ng/ml): 25.21, Standard deviation: 0.174, CV(%): 6.9,
- Sample 1: n=24, Mean(ng/ml): 6.72, Standard deviation: 0.457, CV(%): 6.8
- Sample 2: n=24, Mean(ng/ml): 13.68, Standard deviation: 0.985, CV(%): 7.2
- Sample 3: n=24, Mean(ng/ml): 26.81, Standard deviation: 2.12, CV(%): 7.9
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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-
: "Neutrophil serine proteases and their endogenous inhibitors in coronary artery ectasia patients." in: Anatolian journal of cardiology, Vol. 16, Issue 1, pp. 23-8, (2016) (PubMed).
: "Disequilibrium of Blood Coagulation and Fibrinolytic System in Patients With Coronary Artery Ectasia." in: Medicine, Vol. 95, Issue 8, pp. e2779, (2016) (PubMed).
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: "Neutrophil serine proteases and their endogenous inhibitors in coronary artery ectasia patients." in: Anatolian journal of cardiology, Vol. 16, Issue 1, pp. 23-8, (2016) (PubMed).
-
- 抗原 See all alpha 2 Macroglobulin (A2M) ELISA试剂盒
- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
- 别名
- A2M (A2M 产品)
- 别名
- A2MD ELISA Kit, CPAMD5 ELISA Kit, FWP007 ELISA Kit, S863-7 ELISA Kit, A2MAC1 ELISA Kit, A2m1 ELISA Kit, A2maa ELISA Kit, Mam ELISA Kit, A2mp ELISA Kit, A2M ELISA Kit, LOC733429 ELISA Kit, endod ELISA Kit, cpamd5 ELISA Kit, fwp007 ELISA Kit, s863-7 ELISA Kit, a2mb ELISA Kit, endodermin ELISA Kit, Alpha-2-M ELISA Kit, alpha-2-macroglobulin ELISA Kit, pregnancy-zone protein L homeolog ELISA Kit, alpha-2-macroglobulin S homeolog ELISA Kit, pregnancy zone protein ELISA Kit, alpha-2-macroglobulin-like ELISA Kit, A2M ELISA Kit, A2m ELISA Kit, LOC477699 ELISA Kit, pzp.L ELISA Kit, AZL_c00450 ELISA Kit, LOC100349077 ELISA Kit, a2m ELISA Kit, LOC100061656 ELISA Kit, LOC100090399 ELISA Kit, a2m.S ELISA Kit, LOC100469973 ELISA Kit, LOC100595735 ELISA Kit, PZP ELISA Kit, LOC100353095 ELISA Kit, LOC101122940 ELISA Kit, LOC101801552 ELISA Kit, LOC100911545 ELISA Kit
- 背景
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Protein Function: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Background: Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease(AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates(activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Synonyms: Alpha-2-macroglobulin,Alpha-2-M,C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5,A2M,CPAMD5,FWP007,
Full Gene Name: Alpha-2-macroglobulin
Cellular Localisation: Secreted. - 基因ID
- 2
- UniProt
- P01023
- 途径
- Lipid Metabolism
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