Galectin 9 ELISA 试剂盒
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Quick Overview for Galectin 9 ELISA 试剂盒 (ABIN1672900)
抗原
See all Galectin 9 (LGALS9) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 93.8 pg/mL
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原理
- Sandwich Human Galectin-9 / Gal 9 ELISA Kit to quantitate Human LGALS9 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: M1-T355Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) ELISA Kit
LGALS9 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) ELISA KitLGALS9 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum
Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) ELISA KitLGALS9 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) ELISA KitLGALS9 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) ELISA KitLGALS9 适用: 大鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) ELISA KitLGALS9 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) ELISA KitLGALS9 适用: 人 Colorimetric Sandwich ELISA 31.25-2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) ELISA KitLGALS9 适用: 人 Colorimetric Sandwich ELISA 93.8 pg/mL - 6000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.7%, 7.1%, 6.1%
Inter-Assay Precision (CV%): 5.4%, 8.4%, 7% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- Galectin 9 (LGALS9) (Lectin, Galactoside-Binding, Soluble, 9 (LGALS9))
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别名
- LGALS9
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背景
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Background: Galectin-9, also called HUAT or LGALS9A is a protein that in humans is encoded by the LGALS9 gene. This gene is mapped to 17q11.2. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. The protein has N- and C- terminal carbohydrate-binding domains connected by a link peptide.
Gene Full Name: galectin 9
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基因ID
- 3965
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UniProt
- O00182
抗原 See all Galectin 9 (LGALS9) ELISA试剂盒
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