IL-31 ELISA 试剂盒
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- 抗原 See all IL-31 (IL31) ELISA试剂盒
- IL-31 (IL31) (Interleukin 31 (IL31))
- 抗原表位
- AA 24-163
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-31
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: T24-C163 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: T24-C163 - Featured
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Interleukin 31 (IL31) ELISA Kit
IL31 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 31 (IL31) ELISA KitIL31 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 31 (IL31) ELISA KitIL31 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 31 (IL31) ELISA KitIL31 适用: 人 AA 24-164 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 31 (IL31) ELISA KitIL31 适用: 犬 Colorimetric Competition ELISA 50-1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Interleukin 31 (IL31) ELISA KitIL31 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Interleukin 31 (IL31) ELISA KitIL31 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- mouse IL-31 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-31 has been precoated onto 96-well plates. Standards(E.coli, T24-C163) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-31 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-31 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse IL-31 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-31 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 387, Standard deviation: 21.3, CV(%): 5.5
- Sample 2: n=16, Mean(pg/ml): 1228, Standard deviation: 78.6, CV(%): 6.4
- Sample 3: n=16, Mean(pg/ml): 2155, Standard deviation: 99.13, CV(%): 4.6,
- Sample 1: n=24, Mean(pg/ml): 453, Standard deviation: 31.26, CV(%): 6.9
- Sample 2: n=24, Mean(pg/ml): 1396, Standard deviation: 100.5, CV(%): 7.2
- Sample 3: n=24, Mean(pg/ml): 2370, Standard deviation: 128, CV(%): 5.4
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all IL-31 (IL31) ELISA试剂盒
- IL-31 (IL31) (Interleukin 31 (IL31))
- 别名
- IL31 (IL31 产品)
- 别名
- IL-31 ELISA Kit, 1700013B14Rik ELISA Kit, IL31 ELISA Kit, interleukin 31 ELISA Kit, IL31 ELISA Kit, Il31 ELISA Kit
- 背景
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Protein Function: Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. .
Background: Interleukin-31, also known as IL-31 is a protein that in humans is encoded by the IL31 gene. The IL31 gene is mapped to chromosome 12q24.31 and the mouse gene maps to a syntenic region on chromosome 5. IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA and OSMR that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma.
Synonyms: Interleukin-31,IL-31,Il31,
Full Gene Name: Interleukin-31
Cellular Localisation: Secreted. - 基因ID
- 76399
- UniProt
- Q6EAL8
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