Granulin ELISA 试剂盒
Quick Overview for Granulin ELISA 试剂盒 (ABIN1672874)
抗原
See all Granulin (GRN) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1.56 ng/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Progranulin
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: T18-L593 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <50pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: T18-L593
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Granulin (GRN) ELISA Kit
GRN 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granulin (GRN) ELISA KitGRN 适用: 小鼠 Colorimetric Competition ELISA 0.469 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
Granulin (GRN) ELISA KitGRN 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granulin (GRN) ELISA KitGRN 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Granulin (GRN) ELISA KitGRN 适用: 小鼠 AA 18-589 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Granulin (GRN) ELISA KitGRN 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the granulin family.
Tissue Specificity: In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.
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板类型
- Pre-coated
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实验流程
- human Progranulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Progranulin has been precoated onto 96-well plates. Standards(NSO, T18-L593) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Progranulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Progranulin amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human Progranulin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine or human milk to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Progranulin standard solution and each sample is measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 15.4, Standard deviation: 0.986, CV(%): 6.4
- Sample 2: n=16, Mean(ng/ml): 37.7, Standard deviation: 2, CV(%): 5.3
- Sample 3: n=16, Mean(ng/ml): 56, Standard deviation: 2.69, CV(%): 4.8,
- Sample 1: n=24, Mean(ng/ml): 13.6, Standard deviation: 1.02, CV(%): 7.5
- Sample 2: n=24, Mean(ng/ml): 32.8, Standard deviation: 2.69, CV(%): 8.2
- Sample 3: n=24, Mean(ng/ml): 61, Standard deviation: 4.76, CV(%): 7.8
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- Granulin (GRN)
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别名
- GRN
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物质类
- Viral Protein
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背景
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Protein Function: Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
Background: Granulin, also known as Proepithelin(PEPI) is a protein that in humans is encoded by the GRN gene. This gene is mapped to 17q21.31. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.
Synonyms: Granulins,Proepithelin,PEPI,Acrogranin,Glycoprotein of 88 Kda,GP88,Glycoprotein 88,Progranulin,Paragranulin,Granulin-1,Granulin G,Granulin-2,Granulin F,Granulin-3,Granulin B,Granulin-4,Granulin A,Granulin-5,Granulin C,Granulin-6,Granulin D,Granulin-7,Granulin E,GRN,
Full Gene Name: Granulins
Cellular Localisation: Secreted. -
基因ID
- 2896
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UniProt
- P28799
抗原 See all Granulin (GRN) ELISA试剂盒
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