FSTL1 ELISA 试剂盒
(1 reference)Quick Overview for FSTL1 ELISA 试剂盒 (ABIN1672869)
抗原
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human FSTL1
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: M1-I308 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: M1-I308
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Follistatin-Like 1 (FSTL1) ELISA Kit
FSTL1 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Follistatin-Like 1 (FSTL1) ELISA KitFSTL1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Follistatin-Like 1 (FSTL1) ELISA KitWide Range FSTL1 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Follistatin-Like 1 (FSTL1) ELISA KitFSTL1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Follistatin-Like 1 (FSTL1) ELISA KitFSTL1 适用: 人 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Contains 2 EF-hand domains.
Tissue Specificity: Overexpressed in synovial tissues from rheumatoid arthritis. .
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板类型
- Pre-coated
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实验流程
- human FSTL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FSTL1 has been precoated onto 96-well plates. Standards(NSO, M1-I308) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FSTL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FSTL1 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human FSTL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human FSTL1 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.34, Standard deviation: 0.056, CV(%): 4.2
- Sample 2: n=16, Mean(ng/ml): 7.2, Standard deviation: 0.382, CV(%): 5.3
- Sample 3: n=16, Mean(ng/ml): 13.9, Standard deviation: 0.89, CV(%): 6.4,
- Sample 1: n=24, Mean(ng/ml): 1.25, Standard deviation: 0.094, CV(%): 7.5
- Sample 2: n=24, Mean(ng/ml): 7.5, Standard deviation: 0.48, CV(%): 6.4
- Sample 3: n=24, Mean(ng/ml): 14.4, Standard deviation: 1.123, CV(%): 7.8
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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: "Follistatin-like protein 1: a serum biochemical marker reflecting the severity of joint damage in patients with osteoarthritis." in: Arthritis research & therapy, Vol. 13, Issue 6, pp. R193, (2012) (PubMed).
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: "Follistatin-like protein 1: a serum biochemical marker reflecting the severity of joint damage in patients with osteoarthritis." in: Arthritis research & therapy, Vol. 13, Issue 6, pp. R193, (2012) (PubMed).
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- FSTL1 (Follistatin-Like 1 (FSTL1))
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别名
- FSTL1
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背景
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Protein Function: May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin (By similarity). .
Background: Follistatin-related protein 1, also called FSTL1 or FRP is a protein that in humans is encoded by the FSTL1 gene. FSTL1 gene is mapped to chromosome 3q13.33 based on an alignment of FSTL1 sequence with the genomic sequence(GRCh37). This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. This gene may modulate the action of some growth factors on cell proliferation and differentiation. It was suggested that fibroblast-secreted FSTL1 acts on macrophages to increase TNF and IL1B production and IL6 activity.
Synonyms: Follistatin-related protein 1,Follistatin-like protein 1,FSTL1,FRP,
Full Gene Name: Follistatin-related protein 1
Cellular Localisation: Secreted. -
基因ID
- 11167
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UniProt
- Q12841
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