Cathepsin S ELISA 试剂盒
Quick Overview for Cathepsin S ELISA 试剂盒 (ABIN1672865)
抗原
See all Cathepsin S (CTSS) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Cathepsin S
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: Q17-I331&S109-I331 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: Q17-I331&S109-I331
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Cathepsin S (CTSS) ELISA Kit
CTSS 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cathepsin S (CTSS) ELISA KitCTSS 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Cathepsin S (CTSS) ELISA KitCTSS 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathepsin S (CTSS) ELISA KitCTSS 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathepsin S (CTSS) ELISA KitCTSS 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
SensoLyte® 520 Cathepsin S Assay KitFRET Fluorometric
Cathepsin S (CTSS) ELISA KitCTSS 适用: 猕猴 Colorimetric Sandwich ELISA 28-7.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Cathepsin S (CTSS) ELISA KitCTSS 适用: 人 Colorimetric Sandwich ELISA 4 pg/mL-1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Cathepsin S (CTSS) ELISA KitCTSS 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- human Cathepsin S ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cathepsin S has been precoated onto 96-well plates. Standards(NSO, Q17-I331 & S109-I331) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin S is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cathepsin S amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Cathepsin S standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Cathepsin S standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 443, Standard deviation: 23.48, CV(%): 5.3
- Sample 2: n=16, Mean(pg/ml): 1215, Standard deviation: 65.61, CV(%): 5.4
- Sample 3: n=16, Mean(pg/ml): 2611, Standard deviation: 151.44, CV(%): 5.8,
- Sample 1: n=24, Mean(pg/ml): 455, Standard deviation: 27.76, CV(%): 6.1
- Sample 2: n=24, Mean(pg/ml): 1219, Standard deviation: 76.80, CV(%): 6.3
- Sample 3: n=24, Mean(pg/ml): 2659, Standard deviation: 178.15, CV(%): 6.7
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- Cathepsin S (CTSS)
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别名
- CTSS
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背景
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Protein Function: Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond- specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.
Background: Cathepsin S, also known as CTSS, is a protein that in humans is encoded by the CTSS gene. A member of the peptidase C1 family, CTSS gene is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. By fluorescence in situ hybridization(FISH), this gene is mapped to 1q21.3. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.
Synonyms: Cathepsin S,3.4.22.27,CTSS,
Full Gene Name: Cathepsin S
Cellular Localisation: Lysosome. -
基因ID
- 1520
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UniProt
- P25774
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途径
- Activation of Innate immune Response, Toll-Like Receptors Cascades
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