ANGPTL4 ELISA 试剂盒
Quick Overview for ANGPTL4 ELISA 试剂盒 (ABIN1672864)
抗原
See all ANGPTL4 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 625 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ANGPTL4
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: L165-S406 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <20pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: L165-S406
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Angiopoietin-Like 4 (ANGPTL4) ELISA Kit
ANGPTL4 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 小鼠 Colorimetric Competition ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 山羊 Colorimetric Competition ELISA Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 大鼠 Colorimetric Sandwich ELISA 7.8-500 pg/mL Bronchoalveolar Lavage, Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 人 Colorimetric Sandwich ELISA 625 pg/mL - 40000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Contains 1 fibrinogen C-terminal domain.
Tissue Specificity: Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. .
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板类型
- Pre-coated
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实验流程
- human ANGPTL4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ANGPTL4 has been precoated onto 96-well plates. Standards(NSO, L165-S406) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANGPTL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ANGPTL4 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 40000pg/mL, 20000pg/mL, 10000pg/mL, 5000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human ANGPTL4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ANGPTL4 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 6.1, Standard deviation: 0.35, CV(%): 5.7
- Sample 2: n=16, Mean(ng/ml): 12.7, Standard deviation: 0.75, CV(%): 5.9
- Sample 3: n=16, Mean(ng/ml): 25.3, Standard deviation: 1.54, CV(%): 6.1,
- Sample 1: n=24, Mean(ng/ml): 6.7, Standard deviation: 0.52, CV(%): 7.8
- Sample 2: n=24, Mean(ng/ml): 13.8, Standard deviation: 1.12, CV(%): 8.1
- Sample 3: n=24, Mean(ng/ml): 27.5, Standard deviation: 2.28, CV(%): 8.3
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- ANGPTL4 (Angiopoietin-Like 4 (ANGPTL4))
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别名
- ANGPTL4
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背景
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Protein Function: Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation (By similarity). .
Background: Angiopoietin-related protein 4(Angptl4) is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, Angptl4 gene is mapped to 19p13.3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.
Synonyms: Angiopoietin-related protein 4,Angiopoietin-like protein 4,Hepatic fibrinogen/angiopoietin-related protein,HFARP,ANGPTL4,ARP4, HFARP, PGAR,PP1158, PSEC0166, UNQ171/PRO197,
Full Gene Name: Angiopoietin-related protein 4
Cellular Localisation: Secreted . Secreted, extracellular space, extracellular matrix . The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4 (By similarity).. -
基因ID
- 51129
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UniProt
- Q9BY76
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途径
- Regulation of Lipid Metabolism by PPARalpha
抗原 See all ANGPTL4 ELISA试剂盒
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