PROCR ELISA 试剂盒
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- 抗原 See all PROCR ELISA试剂盒
- PROCR (Protein C Receptor, Endothelial (PROCR))
- 抗原表位
- AA 18-210
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 312-20000 pg/mL
- 最低检测浓度
- 312 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Human EPCR
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: S18-S210 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: S18-S210 - Featured
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- Discover our top product PROCR ELISA Kit
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Protein C Receptor, Endothelial (PROCR) ELISA Kit
PROCR 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Protein C Receptor, Endothelial (PROCR) ELISA KitPROCR 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Protein C Receptor, Endothelial (PROCR) ELISA KitPROCR 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Protein C Receptor, Endothelial (PROCR) ELISA KitSmall Sample PROCR 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Protein C Receptor, Endothelial (PROCR) ELISA KitPROCR 适用: 小鼠 AA 18-214 Colorimetric Sandwich ELISA
Protein C Receptor, Endothelial (PROCR) ELISA KitPROCR 适用: 人 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney.
- 板类型
- Pre-coated
- 实验流程
- human EPCR ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EPCR has been precoated onto 96-well plates. Standards(NSO, S18-S210) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPCR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human EPCR amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human EPCR standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human EPCR standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 2.21, Standard deviation: 0.095, CV(%): 4.3
- Sample 2: n=16, Mean(ng/ml): 8.45, Standard deviation: 0.313, CV(%): 3.7
- Sample 3: n=16, Mean(ng/ml): 14.4, Standard deviation: 0.691, CV(%): 4.8,
- Sample 1: n=24, Mean(ng/ml): 2.13, Standard deviation: 0.124, CV(%): 5.8
- Sample 2: n=24, Mean(ng/ml): 8.79, Standard deviation: 0.466, CV(%): 5.3
- Sample 3: n=24, Mean(ng/ml): 13.7, Standard deviation: 1.04, CV(%): 7.6
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all PROCR ELISA试剂盒
- PROCR (Protein C Receptor, Endothelial (PROCR))
- 别名
- PROCR (PROCR 产品)
- 别名
- PROCR ELISA Kit, CCCA ELISA Kit, CCD41 ELISA Kit, EPCR ELISA Kit, AI325044 ELISA Kit, Ccca ELISA Kit, Ccd41 ELISA Kit, Epcr ELISA Kit, protein C receptor ELISA Kit, protein C receptor, endothelial ELISA Kit, PROCR ELISA Kit, Procr ELISA Kit
- 背景
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Protein Function: Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex, plays a role in the protein C pathway controlling blood coagulation.
Background: Endothelial protein C receptor(EPCR) also known as CCCA or PROCR is a protein that in humans is encoded by the PROCR gene. PRPCR gene is mapped to 20q11.2 by radiation hybrid analysis and FISH. The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.
Synonyms: Endothelial protein C receptor,Activated protein C receptor,APC receptor,Endothelial cell protein C receptor,CD201,PROCR,EPCR,
Full Gene Name: Endothelial protein C receptor
Cellular Localisation: Membrane, Single-pass type I membrane protein. - 基因ID
- 10544
- UniProt
- Q9UNN8
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