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Endothelin 1 ELISA 试剂盒

EDN1 适用: 人 AA 53-73 Colorimetric Sandwich ELISA 3.9-250 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
产品编号 ABIN1672856
发货至: 中国
  • 抗原 See all Endothelin 1 (EDN1) ELISA试剂盒
    Endothelin 1 (EDN1)
    抗原表位
    AA 53-73
    适用
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    3.9-250 pg/mL
    最低检测浓度
    3.9 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Endothelin
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: human peptide
    Immunogen sequence: C53-W73
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    预测反应
    Hamster
    灵敏度
    <0.5pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: human peptide
    Immunogen sequence: C53-W73
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Sequence similarities: Belongs to the endothelin/sarafotoxin family.

    Tissue Specificity: Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. .

    板类型
    Pre-coated
    实验流程
    human Endothelin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Endothelin has been precoated onto 96-well plates. Standards(human peptide, C53-W73) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Endothelin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Endothelin amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 16.5pg/mL, 7.8pg/mL, 3.9pg/mL human Endothelin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Endothelin standard solution and each sample is measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 25.1, Standard deviation: 1.41, CV(%): 5.6
    • Sample 2: n=16, Mean(pg/ml): 85.4, Standard deviation: 3.76, CV(%): 4.4
    • Sample 3: n=16, Mean(pg/ml): 146, Standard deviation: 8.03, CV(%): 5.5,
    • Sample 1: n=24, Mean(pg/ml): 23.2, Standard deviation: 1.79, CV(%): 7.7
    • Sample 2: n=24, Mean(pg/ml): 84.3, Standard deviation: 6.07, CV(%): 7.2
    • Sample 3: n=24, Mean(pg/ml): 143, Standard deviation: 8.72, CV(%): 6.1
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • Liu, Zhu, Zhou, Wei, Long, Chen, Ling, Ge, Zhuo: "Endoplasmic reticulum stress promotes amyloid-beta peptides production in RGC-5 cells." in: Cell stress & chaperones, Vol. 19, Issue 6, pp. 827-35, (2014) (PubMed).

    Peng, Dai, Ji, Dai: "The separate roles of endothelin receptors participate in remodeling of matrix metalloproteinase and connexin 43 of cardiac fibroblasts in maladaptive response to isoproterenol." in: European journal of pharmacology, Vol. 634, Issue 1-3, pp. 101-6, (2010) (PubMed).

  • 抗原 See all Endothelin 1 (EDN1) ELISA试剂盒
    Endothelin 1 (EDN1)
    别名
    EDN1 (EDN1 产品)
    别名
    et1 ELISA Kit, et-1 ELISA Kit, Edn1 ELISA Kit, EDN ELISA Kit, ET-1 ELISA Kit, ET1 ELISA Kit, PPET1 ELISA Kit, HDLCQ7 ELISA Kit, preproET ELISA Kit, Et1 ELISA Kit, fb14d01 ELISA Kit, wu:fb14d01 ELISA Kit, PPET-1 ELISA Kit, EDN1 ELISA Kit, edn1 ELISA Kit, edn1-a ELISA Kit, edn1-b ELISA Kit, endothelin 1 ELISA Kit, endothelin 1 S homeolog ELISA Kit, endothelin 1 L homeolog ELISA Kit, edn1 ELISA Kit, EDN1 ELISA Kit, Edn1 ELISA Kit, edn1.S ELISA Kit, edn1.L ELISA Kit
    背景

    Protein Function: Endothelins are endothelium-derived vasoconstrictor peptides.

    Background: The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure(hypertension) and heart disease.

    Synonyms: Endothelin-1,Preproendothelin-1,PPET1,Endothelin-1,ET-1,Big endothelin-1,EDN1,

    Full Gene Name: Endothelin-1

    Cellular Localisation: Secreted.
    基因ID
    1906
    UniProt
    P05305
    途径
    Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size
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