Soluble E-Selectin ELISA 试剂盒
Quick Overview for Soluble E-Selectin ELISA 试剂盒 (ABIN1672849)
抗原
See all Soluble E-Selectin (sE-Selectin) products抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat sE-Selectin
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: W22-P494 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: W22-P494
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Selectin E (Soluble) (sE-Selectin) ELISA Kit
sE-Selectin 适用: 小鼠 AA 22-557 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (citrate), Plasma (heparin), Serum
Selectin E (Soluble) (sE-Selectin) ELISA KitsE-Selectin 适用: 人 Colorimetric Sandwich ELISA 780-50000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Selectin E (Soluble) (sE-Selectin) ELISA KitsE-Selectin 适用: 大鼠 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
Selectin E (Soluble) (sE-Selectin) ELISA KitsE-Selectin 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (heparin - citrate), Serum
Selectin E (Soluble) (sE-Selectin) ELISA KitsE-Selectin 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (heparin - citrate), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the selectin/LECAM family.
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板类型
- Pre-coated
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实验流程
- rat E-Selectin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for E-Selectin has been precoated onto 96-well plates. Standards(NSO, W22-P494) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for E-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat E-Selectin amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat E-Selectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat E-Selectin standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.1, Standard deviation: 0.044, CV(%): 4
- Sample 2: n=16, Mean(ng/ml): 2.5, Standard deviation: 0.16, CV(%): 6.4
- Sample 3: n=16, Mean(ng/ml): 6.3, Standard deviation: 0.328, CV(%): 5.2,
- Sample 1: n=24, Mean(ng/ml): 0.9, Standard deviation: 0.044, CV(%): 4.9
- Sample 2: n=24, Mean(ng/ml): 2.3, Standard deviation: 0.168, CV(%): 7.3
- Sample 3: n=24, Mean(ng/ml): 7, Standard deviation: 0.448, CV(%): 6.4
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- Soluble E-Selectin (sE-Selectin) (Selectin E (Soluble) (sE-Selectin))
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别名
- sE-Selectin
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背景
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Protein Function: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis (By similarity). .
Background: E-selectin, also called endothelial-leukocyte adhesion molecule-1(ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils.1 An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions.2 E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells(HPCs) into bone marrow(BM).3 A structurally and functionally related group of genes, lymph node homing receptor(LHR), granule membrane protein 140(GMP-140), and endothelial leukocyte adhesion molecule 1(ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1.4
Synonyms: E-selectin,CD62 antigen-like family member E,Endothelial leukocyte adhesion molecule 1,ELAM-1,Leukocyte-endothelial cell adhesion molecule 2,LECAM2,CD62E,Sele,Elam-1,
Full Gene Name: E-selectin
Cellular Localisation: Cell membrane, Single-pass type I membrane protein. -
基因ID
- 25544
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UniProt
- P98105
抗原 See all Soluble E-Selectin (sE-Selectin) products
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