CD103, Intestinal Lymphocyte (HML1) 105-150 kDa ELISA 试剂盒
The 人 CD103, Intestinal Lymphocyte (HML1) 105-150 kDa ELISA Kit (ABIN1672842) is a Colorimetric ELISA Kit designed to quantify 人 CD103, Intestinal Lymphocyte (HML1) 105-150 kDa. This ELISA Kit has been cited in 3 publications.
(3 references)
产品编号 ABIN1672842
发货至:
中国
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Quick Overview for CD103, Intestinal Lymphocyte (HML1) 105-150 kDa ELISA 试剂盒 (ABIN1672842)
抗原
CD103, Intestinal Lymphocyte (HML1) 105-150 kDa
抗原表位
AA 33-251
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
15.6 pg/mL - 1000 pg/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Cell Lysate, Urine
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最低检测浓度
- 15.6 pg/mL
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原理
- Sandwich Human Tissue Factor/F3 ELISA Kit to quantitate Human F3 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) , Cell lysates, and urine.
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: S33-E251Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.5%, 6.6%, 7.6%
Inter-Assay Precision (CV%): 5.5%, 6.9%, 8.3% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "Evaluation of venous thrombosis and tissue factor in epithelial ovarian cancer." in: Gynecologic oncology, Vol. 146, Issue 1, pp. 146-152, (2017) (PubMed).
: "Edaravone rescues the lung by inhibiting lipid peroxidation and pro-inflammatory cytokines in a rat model." in: Chinese medical journal, Vol. 127, Issue 5, pp. 968-70, (2014) (PubMed).
: "Proteomic screening of anaerobically regulated promoters from Salmonella and its antitumor applications." in: Molecular & cellular proteomics : MCP, Vol. 10, Issue 6, pp. M111.009399, (2011) (PubMed).
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- CD103, Intestinal Lymphocyte (HML1) 105-150 kDa
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别名
- Tissue factor/F2
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背景
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Background: Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. An incorrect synonym is thromboplastin. the F3 gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.
Gene Full Name: coagulation factor III, tissue factor
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基因ID
- 2152
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UniProt
- P13726
抗原
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