CXCL5 ELISA 试剂盒
Quick Overview for CXCL5 ELISA 试剂盒 (ABIN1672835)
抗原
See all CXCL5 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CXCL5/ENA-78
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: V45-A118 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: V45-A118
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Chemokine (C-X-C Motif) Ligand 5 (CXCL5) ELISA Kit
CXCL5 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 5 (CXCL5) ELISA KitCXCL5 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
Chemokine (C-X-C Motif) Ligand 5 (CXCL5) ELISA KitCXCL5 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 5 (CXCL5) ELISA KitCXCL5 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 5 (CXCL5) ELISA KitCXCL5 适用: 人 AA 37-114 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Chemokine (C-X-C Motif) Ligand 5 (CXCL5) ELISA KitCXCL5 适用: 大鼠 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Chemokine (C-X-C Motif) Ligand 5 (CXCL5) ELISA KitCXCL5 适用: 大鼠 Colorimetric Sandwich ELISA 20-6000 pg/mL Cell Culture Supernatant, Plasma, Serum
Chemokine (C-X-C Motif) Ligand 5 (CXCL5) ELISA KitCXCL5 适用: 大鼠 AA 38-130 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 5 (CXCL5) ELISA KitCXCL5 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
Chemokine (C-X-C Motif) Ligand 5 (CXCL5) ELISA KitCXCL5 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- mouse CXCL5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL5 has been precoated onto 96-well plates. Standards(E.coli, V45-A118) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL5 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse CXCL5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CXCL5 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 89, Standard deviation: 4.54, CV(%): 5.1
- Sample 2: n=16, Mean(pg/ml): 238, Standard deviation: 13.3, CV(%): 5.6
- Sample 3: n=16, Mean(pg/ml): 496, Standard deviation: 18.9, CV(%): 3.8,
- Sample 1: n=24, Mean(pg/ml): 103, Standard deviation: 7.42, CV(%): 7.2
- Sample 2: n=24, Mean(pg/ml): 276, Standard deviation: 20.7, CV(%): 7.5
- Sample 3: n=24, Mean(pg/ml): 513, Standard deviation: 32.8, CV(%): 6.4
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))
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别名
- CXCL5
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背景
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Protein Function: May participate in the recruitment of inflammatory cells by injured or infected tissue. GCP-2(1-78) and, more potent, GCP- 2(9-78) attract neutrophils and are involved in neutrophil activation. .
Background: C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78(ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.
Synonyms: C-X-C motif chemokine 5,Cytokine LIX,Small-inducible cytokine B5,GCP-2(1-78),GCP-2(9-78),Cxcl5,Scyb5,
Full Gene Name: C-X-C motif chemokine 5
Cellular Localisation: Secreted. -
基因ID
- 20311
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UniProt
- P50228
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity
抗原 See all CXCL5 ELISA试剂盒
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