COMP ELISA 试剂盒
Quick Overview for COMP ELISA 试剂盒 (ABIN1672831)
抗原
See all COMP ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human COMP
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: Q21-A757 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: Q21-A757
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Cartilage Oligomeric Matrix Protein (COMP) ELISA Kit
COMP 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cartilage Oligomeric Matrix Protein (COMP) ELISA KitCOMP 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cartilage Oligomeric Matrix Protein (COMP) ELISA KitCOMP 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cartilage Oligomeric Matrix Protein (COMP) ELISA KitCOMP 适用: 犬 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cartilage Oligomeric Matrix Protein (COMP) ELISA KitCOMP 适用: 马 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cartilage Oligomeric Matrix Protein (COMP) ELISA KitSmall Sample COMP 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cartilage Oligomeric Matrix Protein (COMP) ELISA KitCOMP 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the thrombospondin family.
Tissue Specificity: Abundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. Increased amounts are produced during late stages of osteoarthritis in the area adjacent to the main defect. .
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板类型
- Pre-coated
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实验流程
- human COMP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for COMP has been precoated onto 96-well plates. Standards(NSO, Q21-A757) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for COMP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human COMP amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human COMP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human COMP standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.02, Standard deviation: 0.052, CV(%): 5.1
- Sample 2: n=16, Mean(ng/ml): 3.12, Standard deviation: 0.131, CV(%): 4.2
- Sample 3: n=16, Mean(ng/ml): 6.19, Standard deviation: 0.254, CV(%): 4.1,
- Sample 1: n=24, Mean(ng/ml): 1.13, Standard deviation: 0.07, CV(%): 6.2
- Sample 2: n=24, Mean(ng/ml): 3.08, Standard deviation: 0.166, CV(%): 5.4
- Sample 3: n=24, Mean(ng/ml): 6.23, Standard deviation: 0.324, CV(%): 5.2
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- COMP (Cartilage Oligomeric Matrix Protein (COMP))
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别名
- COMP
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背景
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Protein Function: May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity). .
Background: Cartilage oligomeric matrix protein is a protein that in humans is encoded by the COMP gene. The sequences of rat and bovine COMP indicate that it is a member of the thrombospondin gene family. By Southern blot analysis of a somatic cell hybrid DNA panel and by isotopic in situ hybridization, human COMP gene was mapped to 19p13.1, and the murine COMP gene was mapped to the central region of mouse chromosome 8 by use of an interspecific backcross mapping panel. COMP is a marker of cartilage turnover.
Synonyms: Cartilage oligomeric matrix protein,COMP,Thrombospondin-5,TSP5,COMP,
Full Gene Name: Cartilage oligomeric matrix protein
Cellular Localisation: Secreted, extracellular space, extracellular matrix. -
基因ID
- 1311
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UniProt
- P49747
抗原 See all COMP ELISA试剂盒
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