PTX3 ELISA 试剂盒
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Quick Overview for PTX3 ELISA 试剂盒 (ABIN1672819)
抗原
See all PTX3 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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原理
- Sandwich Human PTX3/Pentraxin 3 ELISA Kit to quantitate Human PTX3 in cell culture supernatants, serum, plasma (EDTA) and saliva.
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: E18-S381Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Pentraxin 3 (PTX3) ELISA Kit
PTX3 适用: 人 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pentraxin 3 (PTX3) ELISA KitPTX3 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Pentraxin 3 (PTX3) ELISA KitPTX3 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Pentraxin 3 (PTX3) ELISA KitPTX3 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Pentraxin 3 (PTX3) ELISA KitPTX3 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Pentraxin 3 (PTX3) ELISA KitPTX3 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Pentraxin 3 (PTX3) ELISA KitPTX3 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Pentraxin 3 (PTX3) ELISA KitPTX3 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Pentraxin 3 (PTX3) ELISA KitPTX3 适用: 人 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate
Pentraxin 3 (PTX3) ELISA KitPTX3 适用: 小鼠 AA 18-381 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 6.9%, 5%, 6.9%
Inter-Assay Precision (CV%): 5.4%, 6.9%, 6.9% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "Sequential Measurements of Pentraxin 3 Serum Levels in Patients with Ventilator-Associated Pneumonia: A Nested Case-Control Study." in: The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale, Vol. 2018, pp. 4074169, (2018) (PubMed).
: "The Relationship Between Serum Pentraxine 3 Levels and Hematological Markers in Patients With Rheumatoid Arthritis." in: Archives of rheumatology, Vol. 33, Issue 1, pp. 39-44, (2018) (PubMed).
: "Pentraxin 3 as a clinical marker in children with lower respiratory tract infection." in: Pediatric pulmonology, Vol. 51, Issue 1, pp. 42-8, (2016) (PubMed).
: "Comparative evaluation of pentraxin 3 levels in GCF during canine retraction with active tieback and NiTi coil spring: An in vivo study." in: Journal of orthodontic science, Vol. 5, Issue 2, pp. 52-6, (2016) (PubMed).
: "Serum Copeptin, Pentraxin 3, Anti-Mullerian Hormone Levels With Echocardiography and Carotid Artery Intima-Media Thickness in Adolescents With Polycystic Ovary Syndrome." in: Journal of clinical medicine research, Vol. 7, Issue 12, pp. 989-94, (2015) (PubMed).
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- PTX3 (Pentraxin 3 (PTX3))
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别名
- PTX3
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背景
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Background: PTX3 (Pentraxin 3) is a member of the pentraxin superfamily. This super family characterized by cyclic multimeric structure. The PTX3 gene is mapped to 3q25.32. The predicted 381-amino acid PTX3 protein has homology to the pentraxin protein family. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. PTX3 is effective in preventing CMV infection and reactivation, as well as subsequent Aspergillus infection. PTX3 activates the classical pathway of complementactivation and facilitates pathogen recognition by macrophages and DCs.
Gene Full Name: pentraxin 3
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基因ID
- 5806
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UniProt
- P26022
抗原 See all PTX3 ELISA试剂盒
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