GDF15 ELISA 试剂盒
Quick Overview for GDF15 ELISA 试剂盒 (ABIN1672794)
抗原
See all GDF15 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human GDF-15
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: CHO
Immunogen sequence: A197-I308 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: CHO
Immunogen sequence: A197-I308
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Growth Differentiation Factor 15 (GDF15) ELISA Kit
GDF15 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 大鼠 Colorimetric Competition ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 大鼠 AA 189-303 Colorimetric Sandwich ELISA
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 小鼠 AA 189-303 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 人 Colorimetric Sandwich ELISA 15.625-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the TGF-beta family.
Tissue Specificity: Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.
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板类型
- Pre-coated
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实验流程
- human GDF-15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GDF-15 has been precoated onto 96-well plates. Standards(CHO, A197-I308) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDF-15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GDF-15 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human GDF-15 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human GDF-15 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 104, Standard deviation: 2.5, CV(%): 2.4
- Sample 2: n=16, Mean(pg/ml): 735, Standard deviation: 27.93, CV(%): 3.8
- Sample 3: n=16, Mean(pg/ml): 1518, Standard deviation: 68.31, CV(%): 4.5,
- Sample 1: n=24, Mean(pg/ml): 121, Standard deviation: 6.897, CV(%): 5.7
- Sample 2: n=24, Mean(pg/ml): 757, Standard deviation: 46.18, CV(%): 6.1
- Sample 3: n=24, Mean(pg/ml): 1526, Standard deviation: 112.9, CV(%): 7.4
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- GDF15 (Growth Differentiation Factor 15 (GDF15))
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别名
- GDF15
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背景
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Background: GDF-15(Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.
Synonyms: Growth/differentiation factor 15,GDF-15,Macrophage inhibitory cytokine 1,MIC-1,NSAID-activated gene 1 protein,NAG-1,NSAID-regulated gene 1 protein,NRG-1,Placental TGF-beta,Placental bone morphogenetic protein,Prostate differentiation factor,GDF15,MIC1, PDF, PLAB, PTGFB,
Full Gene Name: Growth/differentiation factor 15
Cellular Localisation: Secreted. -
基因ID
- 9518
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UniProt
- Q99988
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途径
- SARS-CoV-2 Protein Interactome
抗原 See all GDF15 ELISA试剂盒
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