CXCL13 ELISA 试剂盒
Quick Overview for CXCL13 ELISA 试剂盒 (ABIN1672786)
抗原
See all CXCL13 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CXCL13/BLC
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: I22-A109 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: I22-A109
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Chemokine (C-X-C Motif) Ligand 13 (CXCL13) ELISA Kit
CXCL13 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) ELISA KitCXCL13 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) ELISA KitCXCL13 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) ELISA KitCXCL13 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) ELISA KitCXCL13 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) ELISA KitCXCL13 适用: 人 AA 23-94 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) ELISA KitCXCL13 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) ELISA KitCXCL13 适用: 人, 猕猴 Colorimetric Sandwich ELISA 12-3000 pg/mL Cell Culture Supernatant, Plasma, Serum
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) ELISA KitCXCL13 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) ELISA KitCXCL13 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Tissue Specificity: Found in spleen (B-cell-rich zone or follicles), Peyer patches (strongest within germinal centers and extending to the mantle zone) and lymph nodes (in reticular pattern in follicles).
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板类型
- Pre-coated
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实验流程
- mouse CXCL13 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL13 has been precoated onto 96-well plates. Standards(E.coli, I22-A109) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL13 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse CXCL13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CXCL13 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 52.8, Standard deviation: 3.54, CV(%): 6.7
- Sample 2: n=16, Mean(pg/ml): 121, Standard deviation: 5.08, CV(%): 4.2
- Sample 3: n=16, Mean(pg/ml): 462, Standard deviation: 19.9, CV(%): 4.3,
- Sample 1: n=24, Mean(pg/ml): 54.1, Standard deviation: 4.44, CV(%): 8.2
- Sample 2: n=24, Mean(pg/ml): 116, Standard deviation: 8.47, CV(%): 7.3
- Sample 3: n=24, Mean(pg/ml): 446, Standard deviation: 36.1, CV(%): 8.1
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- CXCL13 (Chemokine (C-X-C Motif) Ligand 13 (CXCL13))
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别名
- CXCL13
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背景
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Protein Function: Strongly chemotactic for B-lymphocytes, weakly for spleen monocytes and macrophages but no chemotactic activity for granulocytes. Binds to BLR1/CXCR5. May play a role in directing the migration of B-lymphocytes to follicles in secondary lymphoid organs.
Background: C-X-C motif chemokine 13(CXCL13), also known as B lymphocyte chemoattractant(BLC), is a protein that in humans is encoded by theCXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family, and it was located on sequence maps to 4q21, near the genes encoding most CXC chemokines. In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the T-cell. Hence, expression of CXCL13 in T-cell lymphomas, such as Angioimmunoblastic T-cell Lymphoma, is thought to reflect a germinal center origin of the neoplastic T-cells.
Synonyms: C-X-C motif chemokine 13,B lymphocyte chemoattractant,CXC chemokine BLC,Small-inducible cytokine B13,Cxcl13,Blc, Scyb13,
Full Gene Name: C-X-C motif chemokine 13
Cellular Localisation: Secreted. -
基因ID
- 55985
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UniProt
- O55038
抗原 See all CXCL13 ELISA试剂盒
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