CD86 ELISA 试剂盒
Quick Overview for CD86 ELISA 试剂盒 (ABIN1672778)
抗原
See all CD86 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 62.5 pg/mL
-
原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CD86/B7-2
-
品牌
- PicoKine™
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: NSO
Immunogen sequence: V29-I250 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- <10pg/mL
-
试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
-
免疫原
-
Expression system for standard: NSO
Immunogen sequence: V29-I250
-
-
CD86 (CD86) ELISA Kit
CD86 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
CD86 (CD86) ELISA KitCD86 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD86 (CD86) ELISA KitCD86 适用: Cow Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
CD86 (CD86) ELISA KitCD86 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
CD86 (CD86) ELISA KitCD86 适用: 小鼠 AA 26-245 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
CD86 (CD86) ELISA KitCD86 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum
CD86 (CD86) ELISA KitCD86 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum
-
-
应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
-
板类型
- Pre-coated
-
实验流程
- rat CD86 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD86 has been precoated onto 96-well plates. Standards(NSO, V29-I250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD86 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CD86 amount of sample captured in plate.
-
实验流程
-
Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat CD86 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat CD86 standard solution and each sample be measured in duplicate.
-
实验精密度
-
- Sample 1: n=16, Mean(ng/ml): 0.21, Standard deviation: 0.010, CV(%): 4.8
- Sample 2: n=16, Mean(ng/ml): 1.44, Standard deviation: 0.102, CV(%): 7.1
- Sample 3: n=16, Mean(ng/ml): 2.59, Standard deviation: 0.161, CV(%): 6.2,
- Sample 1: n=24, Mean(ng/ml): 0.23, Standard deviation: 0.013, CV(%): 5.8
- Sample 2: n=24, Mean(ng/ml): 1.47, Standard deviation: 0.118, CV(%): 8.0
- Sample 3: n=24, Mean(ng/ml): 2.46, Standard deviation: 0.182, CV(%): 7.4
-
限制
- 仅限研究用
-
-
-
注意事项
- Avoid multiple freeze-thaw cycles.
-
储存条件
- -20 °C,4 °C
-
储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
-
有效期
- 12 months
-
-
- CD86
-
别名
- CD86
-
背景
-
Background: Cluster of Differentiation 86(also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.
Synonyms: B7-2 ,Cd86 ,
Full Gene Name: CD86 Molecule
-
基因ID
- 56822
-
UniProt
- Q62810
-
途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
抗原 See all CD86 ELISA试剂盒
-
