IL-20 ELISA 试剂盒
(4 references)-
- 抗原 See all IL-20 (IL20) ELISA试剂盒
- IL-20 (IL20) (Interleukin 20 (IL20))
- 抗原表位
- AA 25-176
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-20
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: L25-E176 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: L25-E176 - Featured
- Discover our best selling IL20 ELISA Kit
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- Discover our top product IL20 ELISA Kit
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Interleukin 20 (IL20) ELISA Kit
IL20 适用: 人 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 20 (IL20) ELISA KitIL20 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 20 (IL20) ELISA KitIL20 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 20 (IL20) ELISA KitIL20 适用: 小鼠 AA 25-176 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 20 (IL20) ELISA KitIL20 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the IL-10 family.
Tissue Specificity: Expressed in most tissues and five major cell types: epithelial cells (primarily skin, buccal mucosa, tongue, nasal mucosa, lung, ureter, breast, prostate, fallopian tube, and adrenal gland), myoepithelial cells (mainly prostate), endothelial cells (mainly in small vessels or capillaries), macrophages, and skeletal muscle. Isoform 2 was detected in the lung tissue only. .
- 板类型
- Pre-coated
- 实验流程
- human IL-20 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-20 has been precoated onto 96-well plates. Standards(E.coli, L25-E176) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-20 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-20 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IL-20 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-20 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 548, Standard deviation: 35.62, CV(%): 6.5
- Sample 2: n=16, Mean(pg/ml): 1202, Standard deviation: 84.14, CV(%): 7
- Sample 3: n=16, Mean(pg/ml): 2279, Standard deviation: 104.8, CV(%): 4.6,
- Sample 1: n=24, Mean(pg/ml): 623, Standard deviation: 45.5, CV(%): 7.3
- Sample 2: n=24, Mean(pg/ml): 1560, Standard deviation: 117, CV(%): 7.5
- Sample 3: n=24, Mean(pg/ml): 2533, Standard deviation: 142, CV(%): 5.6
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Baicalin attenuates brain edema in a rat model of intracerebral hemorrhage." in: Inflammation, Vol. 37, Issue 1, pp. 107-15, (2014) (PubMed).
: "Antitumor activity of the protein and small molecule component fractions from Agrocybe aegerita through enhancement of cytokine production." in: Journal of medicinal food, Vol. 17, Issue 4, pp. 439-46, (2014) (PubMed).
: "E-selectin rs5361 and FCGR2A rs1801274 variants were associated with increased risk of gastric cancer in a Chinese population." in: Molecular carcinogenesis, Vol. 51, Issue 8, pp. 597-607, (2012) (PubMed).
: "A rat model for stable chronic obstructive pulmonary disease induced by cigarette smoke inhalation and repetitive bacterial infection." in: Biological & pharmaceutical bulletin, Vol. 35, Issue 10, pp. 1752-60, (2012) (PubMed).
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: "Baicalin attenuates brain edema in a rat model of intracerebral hemorrhage." in: Inflammation, Vol. 37, Issue 1, pp. 107-15, (2014) (PubMed).
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- 抗原 See all IL-20 (IL20) ELISA试剂盒
- IL-20 (IL20) (Interleukin 20 (IL20))
- 别名
- IL20 (IL20 产品)
- 别名
- IL-20 ELISA Kit, IL10D ELISA Kit, ZCYTO10 ELISA Kit, Zcyto10 ELISA Kit, IL20 ELISA Kit, interleukin 20 ELISA Kit, IL20 ELISA Kit, Il20 ELISA Kit
- 背景
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Protein Function: Proinflammatory and angiogenic cytokine that may be involved in epidermal function and psoriasis. Angiogenic and proliferative activities are antagonized by IL10. May act through STAT3. .
Background: Interleukin-20(IL-20) is a protein belonging to the IL-10 family of cytokines. IL-20 is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. By radiation hybrid analysis, the IL20 gene was mapped to chromosome 1q32, where it is tightly linked to the IL10, IL19, and MDA7 genes within a 195-kb region, the IL10 family cytokine cluster. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.
Synonyms: Interleukin-20,IL-20,Cytokine Zcyto10,IL20,ZCYTO10,UNQ852/PRO1801,
Full Gene Name: Interleukin-20
Cellular Localisation: Secreted. - 基因ID
- 50604
- UniProt
- Q9NYY1
- 途径
- Protein targeting to Nucleus
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