CCL24 ELISA 试剂盒
Quick Overview for CCL24 ELISA 试剂盒 (ABIN1672758)
抗原
See all CCL24 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CCL24/Eotaxin-2
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: V27-V119 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: V27-V119
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Chemokine (C-C Motif) Ligand 24 (CCL24) ELISA Kit
CCL24 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 24 (CCL24) ELISA KitCCL24 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 24 (CCL24) ELISA KitCCL24 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 24 (CCL24) ELISA KitCCL24 适用: 人 AA 27-119 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 24 (CCL24) ELISA KitCCL24 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Chemokine (C-C Motif) Ligand 24 (CCL24) ELISA KitCCL24 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Tissue Specificity: Highest expression in jejunum and spleen. Lower levels found in liver and lung. No expression detected in kidney, thymus, brain or testis. .
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板类型
- Pre-coated
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实验流程
- mouse CCL24 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL24 has been precoated onto 96-well plates. Standards(E.coli, V27-V119) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL24 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL24 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse CCL24 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CCL24 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 125, Standard deviation: 6, CV(%): 4.8
- Sample 2: n=16, Mean(pg/ml): 257, Standard deviation: 10.79, CV(%): 4.2
- Sample 3: n=16, Mean(pg/ml): 526, Standard deviation: 33.14, CV(%): 6.3,
- Sample 1: n=24, Mean(pg/ml): 130, Standard deviation: 10.14, CV(%): 7.8
- Sample 2: n=24, Mean(pg/ml): 249, Standard deviation: 15.94, CV(%): 6.4
- Sample 3: n=24, Mean(pg/ml): 518, Standard deviation: 42.99, CV(%): 8.3
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))
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别名
- CCL24
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背景
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Protein Function: Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Binds to CCR3 (By similarity). .
Background: Chemokine(C-C motif) ligand 24(CCL24) also known as myeloid progenitor inhibitory factor 2(MPIF-2) or eosinophil chemotactic protein 2(eotaxin-2) is a protein that in humans is encoded by the CCL24 gene.1 By use of PCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23.2 CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils.3 This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils.
Synonyms: C-C motif chemokine 24,Eosinophil chemotactic protein 2,Eotaxin-2,Small-inducible cytokine A24,Ccl24,Scya24,
Full Gene Name: C-C motif chemokine 24
Cellular Localisation: Secreted. -
基因ID
- 56221
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UniProt
- Q9JKC0
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途径
- Regulation of Actin Filament Polymerization
抗原 See all CCL24 ELISA试剂盒
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