PDGF-AB Heterodimer ELISA 试剂盒
(3 references)-
- 抗原
- PDGF-AB Heterodimer
- 抗原表位
- AA 82-190, AA 87-211
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse PDGF-AB
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: E.coli
Immunogen sequence: (S87-T211)+(S82-T190) - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <3pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: E.coli
Immunogen sequence: (S87-T211)+(S82-T190) - Featured
- Discover our best selling ELISA Kit
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PDGF-AB Heterodimer ELISA Kit
适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
PDGF-AB Heterodimer ELISA Kit适用: 大鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
PDGF-AB Heterodimer ELISA Kit适用: 兔 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
PDGF-AB Heterodimer ELISA Kit适用: Pig Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
PDGF-AB Heterodimer ELISA Kit适用: 绵羊 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
PDGF-AB Heterodimer ELISA Kit适用: 人 AA 87-211 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
PDGF-AB Heterodimer ELISA Kit适用: 大鼠 AA 86-204 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
PDGF-AB Heterodimer ELISA Kit适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
PDGF-AB Heterodimer ELISA Kit适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Localized to vascular smooth muscle cells. Also weakly expressed by cortical interstitial cells but absent in tubules. Up-regulated in areas of renal fibrosis. In mice with unilateral ureteral obstruction, an increased expression in interstitial cells and in some tubules observed after day 4. .
- 板类型
- Pre-coated
- 实验流程
- mouse PDGF-AB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PDGF-AB has been precoated onto 96-well plates. Standards(E.coli, (S87-T211)+(S82-T190)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PDGF-AB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse PDGF-AB amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse PDGF-AB standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse PDGF-AB standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 262, Standard deviation: 12.3, CV(%): 4.7
- Sample 2: n=16, Mean(pg/ml): 524, Standard deviation: 27.8, CV(%): 5.3
- Sample 3: n=16, Mean(pg/ml): 924, Standard deviation: 57.3, CV(%): 6.2,
- Sample 1: n=24, Mean(pg/ml): 280, Standard deviation: 15.4, CV(%): 5.5
- Sample 2: n=24, Mean(pg/ml): 649, Standard deviation: 41.54, CV(%): 6.4
- Sample 3: n=24, Mean(pg/ml): 1107, Standard deviation: 80.8, CV(%): 7.3
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Combination of Controlled Release Platelet-Rich Plasma Alginate Beads and Bone Morphogenetic Protein-2 Genetically Modified Mesenchymal Stem Cells for Bone Regeneration." in: Journal of periodontology, Vol. 87, Issue 4, pp. 470-80, (2017) (PubMed).
: "Cilostazol attenuates intimal hyperplasia in a mouse model of chronic kidney disease." in: PLoS ONE, Vol. 12, Issue 12, pp. e0187872, (2017) (PubMed).
: "Characterization of bone marrow-derived mesenchymal stem cells from dimethyloxallyl glycine-preconditioned mice: Evaluation of the feasibility of dimethyloxallyl glycine as a mobilization agent." in: Molecular medicine reports, Vol. 13, Issue 4, pp. 3498-506, (2016) (PubMed).
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: "Combination of Controlled Release Platelet-Rich Plasma Alginate Beads and Bone Morphogenetic Protein-2 Genetically Modified Mesenchymal Stem Cells for Bone Regeneration." in: Journal of periodontology, Vol. 87, Issue 4, pp. 470-80, (2017) (PubMed).
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- 抗原
- PDGF-AB Heterodimer
- 别名
- PDGF-AB
- 别名
- PDGF-A ELISA Kit, PDGF1 ELISA Kit, platelet derived growth factor subunit A ELISA Kit, PDGFA ELISA Kit
- 背景
-
Protein Function: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. .
Background: The platelet-derived growth factor(PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. The entire B chain of PDGF is highly(96 % ) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus(SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner. PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog(PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22.
Synonyms: Platelet-derived growth factor subunit B,PDGF subunit B,PDGF-2,Platelet-derived growth factor B chain,Platelet-derived growth factor beta polypeptide,Proto-oncogene c-Sis,Pdgfb,Sis,
Full Gene Name: Platelet-derived growth factor subunit B
Cellular Localisation: Secreted. Released by platelets upon wounding.. - 基因ID
- 18591, 18590
- UniProt
- P31240
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