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MMP7 ELISA 试剂盒

Name: MMP7 ELISA 试剂盒
SKU: ABIN1672745
Availability: OutOfStock

This 人 MMP7 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 MMP7 in Cell Culture Supernatant, Serum, Plasma (heparin), Saliva 和 Urine. This ELISA Kit has been cited in 2+ publications.

ELISA

1 image

(2 references)

产品编号 ABIN1672745

发货至: 中国

Datasheet as PDF

Quick Overview for MMP7 ELISA 试剂盒 (ABIN1672745)

抗原

See all MMP7 ELISA试剂盒

MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))

抗原表位

AA 18-267

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

156-10000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Saliva, Urine

Discover our top product MMP7 ELISA Kit

最低检测浓度

156 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MMP-7

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: NSO
Immunogen sequence: L18-K267

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<6pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: NSO
Immunogen sequence: L18-K267
Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA Kit
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Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA Kit
MMP7 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Saliva, Serum, Urine

Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7) ELISA Kit
MMP7 适用: 人, 小鼠, 大鼠 Colorimetric Cell ELISA Cell Culture Cells

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Sequence similarities: Belongs to the peptidase M10A family.

板类型

Pre-coated

实验流程

human MMP-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-7 has been precoated onto 96-well plates. Standards(NSO, L18-K267) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP-7 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MMP-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin), saliva or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MMP-7 standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(ng/ml): 1.37, Standard deviation: 0.073, CV(%): 5.3
- Sample 2: n=16, Mean(ng/ml): 4.75, Standard deviation: 0.2, CV(%): 4.2
- Sample 3: n=16, Mean(ng/ml): 6.34, Standard deviation: 0.241, CV(%): 3.8,
- Sample 1: n=24, Mean(ng/ml): 1.25, Standard deviation: 0.078, CV(%): 6.2
- Sample 2: n=24, Mean(ng/ml): 4.81, Standard deviation: 0.284, CV(%): 5.9
- Sample 3: n=24, Mean(ng/ml): 6.53, Standard deviation: 0.438, CV(%): 6.7
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Sun, Chen, Li, Lei, Xu, Jiang, Zhang, Cao, Ke: "NanoVelcro-captured CTC number concomitant with enhanced serum levels of MMP7 and MMP9 enables accurate prediction of metastasis and poor prognosis in patients with lung adenocarcinoma." in: International journal of nanomedicine, Vol. 12, pp. 6399-6412, (2017) (PubMed).

Kim, Lee, Choi, Yoo, Yang: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." in: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, (2012) (PubMed).
抗原 See all MMP7 ELISA试剂盒

MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))

别名

MMP7

背景

Protein Function: Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. .
Background: Matrix metalloproteinase-7(MMP-7) previously called putative metalloproteinase I(PUMP1) or matrilysin. The PUMP1 gene has been identified through studies of collagenase-related connective-tissue-degrading metalloproteinases produced by human tumors. The PUMP I protein has 267 amino acids and is significantly shorter than stromelysin or collagenase(477 and 469 amino acids, respectively). Matrix metalloproteinases play a crucial role in tumor invasion and metastasis. Matrilysin, a member of the matrix metalloproteinase family, is structurally different from the other matrix metalloproteinases by virtue of the absence of a conserved COOH-terminal protein domain. In addition, matrilysin mRNA is regulated in a specific and distinct manner in normal and malignant tissues. Matrilysin has been shown to correlate with nodal or distant metastasis in colorectal carcinomas, however, its implication in early invasive colorectal carcinomas has not been determined.1 Matrilysin is also a mediator of pulmonary fibrosis and a potential therapeutic target. The standard product used in this kit is recombinant human MMP-7, consisting of 250 amino acids with the molecular mass of 28KDa. The detected MMP-7 includes zymogen and active enzyme.
Synonyms: Matrilysin,3.4.24.23,Matrin,Matrix metalloproteinase-7,MMP-7,Pump-1 protease,Uterine metalloproteinase,MMP7,MPSL1, PUMP1,
Full Gene Name: Matrilysin
Cellular Localisation: Secreted, extracellular space, extracellular matrix.

基因ID

4316

UniProt

P09237

途径

Production of Molecular Mediator of Immune Response