CD25 ELISA 试剂盒
Quick Overview for CD25 ELISA 试剂盒 (ABIN1672737)
抗原
See all CD25 (IL2RA) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 78 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD25/IL-2sR alpha
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
- Expression system for standard: NSO,E22-C213
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <5pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: E22-C213
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Interleukin 2 Receptor, alpha (IL2RA) ELISA Kit
IL2RA 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 小鼠 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: Cow Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 小鼠 AA 22-236 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 人 Soluble Colorimetric Sandwich ELISA 78.13-5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Contains 2 Sushi (CCP/SCR) domains.
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板类型
- Pre-coated
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实验流程
- human CD25 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD25 has been precoated onto 96-well plates. Standards (NSO,E22-C213) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD25 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD25 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human CD25 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human CD25 standard solution and each sample is measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 0.22, Standard deviation: 0.014, CV(%): 6.3
- Sample 2: n=16, Mean(ng/ml): 0.63, Standard deviation: 0.041, CV(%): 6.5
- Sample 3: n=16, Mean(ng/ml): 2.53, Standard deviation: 0.132, CV(%): 5.2,
- Sample 1: n=24, Mean(ng/ml): 0.25, Standard deviation: 0.020, CV(%): 8
- Sample 2: n=24, Mean(ng/ml): 0.71, Standard deviation: 0.052, CV(%): 7.3
- Sample 3: n=24, Mean(ng/ml): 2.36, Standard deviation: 0.191, CV(%): 8.1
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
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别名
- IL2RA
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背景
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Protein Function: Receptor for interleukin-2.
Background: CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. The IL2R gene was mapped to 10p15-p14. CD25 is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
Synonyms: Interleukin-2 receptor subunit alpha,IL-2 receptor subunit alpha,IL-2-RA,IL-2R subunit alpha,IL2-RA,TAC antigen,p55,CD25,IL2RA,
Full Gene Name: Interleukin-2 receptor subunit alpha
Cellular Localisation: Membrane, Single-pass type I membrane protein. -
基因ID
- 3559
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UniProt
- P01589
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途径
- JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
抗原 See all CD25 (IL2RA) ELISA试剂盒
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