IGF2 ELISA 试剂盒
Quick Overview for IGF2 ELISA 试剂盒 (ABIN1672734)
抗原
See all IGF2 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IGF-2
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: A25-E91 -
交叉反应 （详细）
- There is no detectable cross-reactivity with IGF-1.
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预测反应
- Hamster
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灵敏度
- <5pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: A25-E91
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Insulin-Like Growth Factor 2 (IGF2) ELISA Kit
IGF2 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Insulin-Like Growth Factor 2 (IGF2) ELISA KitIGF2 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 2 (IGF2) ELISA KitIGF2 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Insulin-Like Growth Factor 2 (IGF2) ELISA KitIGF2 适用: 人 Colorimetric Sandwich ELISA 7.81 ng/mL - 500 ng/mL Cell Culture Supernatant, Plasma, Serum
Insulin-Like Growth Factor 2 (IGF2) ELISA KitIGF2 适用: 兔 Colorimetric Sandwich ELISA 4.688 ng/mL - 300 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 2 (IGF2) ELISA KitIGF2 适用: 豚鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 2 (IGF2) ELISA KitIGF2 适用: 小鸡 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 2 (IGF2) ELISA KitIGF2 适用: 鸭 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 2 (IGF2) ELISA KitIGF2 适用: 绵羊 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 2 (IGF2) ELISA KitIGF2 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the insulin family.
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板类型
- Pre-coated
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实验流程
- rat IGF-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGF-2 has been precoated onto 96-well plates. Standards(E.coli, A25-E91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IGF-2 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat IGF-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat IGF-2 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 478, Standard deviation: 20.55, CV(%): 4.3
- Sample 2: n=16, Mean(pg/ml): 1536, Standard deviation: 87.55, CV(%): 5.7
- Sample 3: n=16, Mean(pg/ml): 2743, Standard deviation: 126.2, CV(%): 4.6,
- Sample 1: n=24, Mean(pg/ml): 453, Standard deviation: 30.8, CV(%): 6.8
- Sample 2: n=24, Mean(pg/ml): 1737, Standard deviation: 140.7, CV(%): 8.1
- Sample 3: n=24, Mean(pg/ml): 2968, Standard deviation: 222.6, CV(%): 7.5
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- IGF2 (Insulin-Like Growth Factor 2 (IGF2))
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别名
- IGF2
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背景
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Protein Function: The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Background: Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis, prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.
Synonyms: Insulin-like growth factor II,IGF-II,Multiplication-stimulating activity,MSA,Multiplication-stimulating polypeptide,Insulin-like growth factor II,Preptin,Igf2,Igf-2,
Full Gene Name: Insulin-like growth factor II
Cellular Localisation: Secreted. -
基因ID
- 24483
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UniProt
- P01346
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途径
- Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation
抗原 See all IGF2 ELISA试剂盒
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