PCK1 ELISA 试剂盒
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- 抗原 See all PCK1 ELISA试剂盒
- PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.56 ng/mL - 100 ng/mL
- 最低检测浓度
- 1.56 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PCK1 in rat serum, plasma, tissue homogenates, cell lysates and other biological fluids.
- 样品类型
- Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of this index.
- 交叉反应 （详细）
- No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
- 灵敏度
- 0.54 ng/mL
- 组件
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- Pre-coated, ready to use 96-well strip plate
- Standard (freeze dried)
- Standard Diluent
- Detection Reagent A
- Detection Reagent B
- Assay Diluent A
- Assay Diluent B
- TMB
- Stop Solution
- Wash Buffer (30X)
- Plate sealer for 96 wells
- Instruction manual
- 试剂未包括
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- Microplate reader with 450 ± 10nm filter.
- Precision single or multi-channel pipettes and disposable tips.
- Eppendorf Tubes for diluting samples.
- Deionized or distilled water.
- Absorbent paper for blotting the microtiter plate.
- Container for Wash Solution.
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phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) ELISA Kit
PCK1 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 100 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) ELISA KitPCK1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) ELISA KitPCK1 适用: 人 Colorimetric Sandwich ELISA 0.47 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) ELISA KitPCK1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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- 样本量
- 100 μL
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately. - 实验流程
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The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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- Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
- Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
- CV(%) = SD/meanX100
- Intra-assay: CV<10%
- Inter-assay: CV<12%
- 限制
- 仅限研究用
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- 注意事项
- The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
- 注意事项
- The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
- 有效期
- 12 months
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- 抗原 See all PCK1 ELISA试剂盒
- PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
- 别名
- PCK1 (PCK1 产品)
- 别名
- PEPCK-C ELISA Kit, PEPCK1 ELISA Kit, PEPCKC ELISA Kit, PEPCK ELISA Kit, PEPCK-M ELISA Kit, PEPCK2 ELISA Kit, 1810010O14Rik ELISA Kit, 9130022B02Rik ELISA Kit, cb856 ELISA Kit, cb924 ELISA Kit, fj93h11 ELISA Kit, zgc:63869 ELISA Kit, wu:fc51c05 ELISA Kit, wu:fj93h11 ELISA Kit, pepck-c ELISA Kit, pepck1 ELISA Kit, pepckc ELISA Kit, PCK1 ELISA Kit, PPCK1 ELISA Kit, AI265463 ELISA Kit, Pck-1 ELISA Kit, GTP ELISA Kit, PCK ELISA Kit, Pepck ELISA Kit, RATPEPCK ELISA Kit, Ppc1C ELISA Kit, PHOSPHOENOLPYRUVATE CARBOXYKINASE ELISA Kit, T28I19.150 ELISA Kit, T28I19_150 ELISA Kit, phosphoenolpyruvate carboxykinase 1 ELISA Kit, 143299_at ELISA Kit, CG10924 ELISA Kit, CG17725 ELISA Kit, Dmel\\CG17725 ELISA Kit, Dromel_CG17725_FBtr0086701_pepck_mORF ELISA Kit, dPEPCK ELISA Kit, pepck ELISA Kit, PEPC ELISA Kit, PEPCase ELISA Kit, ppc ELISA Kit, phosphoenolpyruvate carboxykinase 1 ELISA Kit, phosphoenolpyruvate carboxykinase 2, mitochondrial ELISA Kit, phosphoenolpyruvate carboxykinase 2 (mitochondrial) ELISA Kit, phosphoenolpyruvate carboxykinase 1 (soluble) ELISA Kit, phosphoenolpyruvate carboxykinase 1 S homeolog ELISA Kit, phosphoenolpyruvate carboxykinase, cytosolic [GTP] ELISA Kit, phosphoenolpyruvate carboxykinase 1, cytosolic ELISA Kit, phosphoenolpyruvate carboxylase 7 ELISA Kit, Phosphoenolpyruvate carboxykinase ELISA Kit, phosphoenolpyruvate carboxylase ELISA Kit, PCK1 ELISA Kit, PCK2 ELISA Kit, Pck2 ELISA Kit, pck1 ELISA Kit, pck1.S ELISA Kit, LOC100634531 ELISA Kit, Pck1 ELISA Kit, pep7 ELISA Kit, Pepck ELISA Kit, LOC107777405 ELISA Kit
- 背景
- Alternative name: PEPCK1, PEPCKC, Cytosolic Phosphoenolpyruvate Carboxykinase, Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
- 基因ID
- 362282
- UniProt
- P07379
- 途径
- Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
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