Phenylalanine Hydroxylase ELISA 试剂盒
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- 抗原 See all Phenylalanine Hydroxylase ELISA试剂盒
- Phenylalanine Hydroxylase
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.56 ng/mL - 100 ng/mL
- 最低检测浓度
- 1.56 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PAH in human tissue homogenates, cell lysates and other biological fluids.
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of this index.
- 交叉反应 （详细）
- No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
- 灵敏度
- 0.56 ng/mL
- 组件
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- Pre-coated, ready to use 96-well strip plate
- Standard (freeze dried)
- Standard Diluent
- Detection Reagent A
- Detection Reagent B
- Assay Diluent A
- Assay Diluent B
- TMB
- Stop Solution
- Wash Buffer (30X)
- Plate sealer for 96 wells
- Instruction manual
- 试剂未包括
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- Microplate reader with 450 ± 10nm filter.
- Precision single or multi-channel pipettes and disposable tips.
- Eppendorf Tubes for diluting samples.
- Deionized or distilled water.
- Absorbent paper for blotting the microtiter plate.
- Container for Wash Solution.
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Phenylalanine Hydroxylase ELISA Kit
适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Phenylalanine Hydroxylase ELISA Kit适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Phenylalanine Hydroxylase ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 23.5 pg/mL - 1500 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Phenylalanine Hydroxylase ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Phenylalanine Hydroxylase ELISA Kit适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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- 样本量
- 100 μL
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately. - 实验流程
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The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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- Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
- Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
- CV(%) = SD/meanX100
- Intra-assay: CV<10%
- Inter-assay: CV<12%
- 限制
- 仅限研究用
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- 注意事项
- The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
- 注意事项
- The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
- 有效期
- 12 months
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- 抗原 See all Phenylalanine Hydroxylase ELISA试剂盒
- Phenylalanine Hydroxylase
- 别名
- PAH (Phenylalanine Hydroxylase 产品)
- 别名
- cb877 ELISA Kit, wu:fb02f10 ELISA Kit, zgc:65981 ELISA Kit, GB18494 ELISA Kit, PAH ELISA Kit, pku ELISA Kit, pku1 ELISA Kit, DDBDRAFT_0206188 ELISA Kit, DDBDRAFT_0231664 ELISA Kit, DDB_0206188 ELISA Kit, DDB_0231664 ELISA Kit, AW106920 ELISA Kit, PH ELISA Kit, PKU ELISA Kit, PKU1 ELISA Kit, phenylalanine-4-hydroxylase ELISA Kit, phenylalanine hydroxylase ELISA Kit, protein henna ELISA Kit, phenylalanine 4-monooxygenase ELISA Kit, pah ELISA Kit, LOC408622 ELISA Kit, PAH ELISA Kit, CpipJ_CPIJ002149 ELISA Kit, Pah ELISA Kit
- 物质类
- Chemical
- 背景
- Alternative name: PKU, PKU1, Phe-4-monooxygenase
- 基因ID
- 5053
- UniProt
- P00439
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