FSH ELISA 试剂盒
Quick Overview for FSH ELISA 试剂盒 (ABIN1569903)
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See all FSH ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 2.47 ng/mL
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原理
- The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of this index in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of this index.
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交叉反应 （详细）
- No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
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灵敏度
- 0.91ng/mL
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组件
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Assay plate: 1×
Standard: 2×
Sample Diluent : 1 × 20 mL
Assay Diluent A: 1 × 10 mL
Assay Diluent B: 1 × 10 mL
Detection Reagent A: 1 × 120 µL
Detection Reagent B: 1 × 120 µL
Wash Buffer(25 x concentrate): 1 × 30 mL
Substrate: 1 × 10 mL
Stop Solution: 1 × 10 mL
Plate sealer for 96 wells: 5×
Instruction: 1× -
试剂未包括
- Microplate reader, Pipettes and pipette tips, EP tube, Deionized or distilled water.
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Follicle-Stimulating Hormone (FSH) ELISA Kit
FSH 适用: 大鼠 Colorimetric Sandwich ELISA 3.13 ng/mL - 200 ng/mL Cell Culture Supernatant, Plasma, Serum
Follicle-Stimulating Hormone (FSH) ELISA KitFSH 适用: 大鼠 Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Plasma, Serum
Follicle-Stimulating Hormone (FSH) ELISA KitFSH 适用: 小鼠 Colorimetric Competition ELISA 2.22 ng/mL - 180 ng/mL Plasma, Serum
Follicle-Stimulating Hormone (FSH) ELISA KitFSH 适用: 大鼠 Colorimetric Competition ELISA 2.344 mIU/mL - 150 mIU/mL Plasma, Serum, Tissue Homogenate
Follicle-Stimulating Hormone (FSH) ELISA KitFSH 适用: 人 Colorimetric Competition ELISA 1.11 mIU/mL - 90 mIU/mL Plasma, Serum
Follicle-Stimulating Hormone (FSH) ELISA KitFSH 适用: 人 Colorimetric Sandwich ELISA 0.78 mIU/mL - 50 mIU/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Follicle-Stimulating Hormone (FSH) ELISA KitFSH 适用: 小鼠 Colorimetric Sandwich ELISA 1.88 ng/mL - 120 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Follicle-Stimulating Hormone (FSH) ELISA KitFSH 适用: 兔 Colorimetric Sandwich ELISA 1.56 mIU/mL - 100 mIU/mL Plasma, Serum, Tissue Homogenate
Follicle-Stimulating Hormone (FSH) ELISA KitFSH 适用: Pig Colorimetric Competition ELISA 23.438 ng/mL - 1500 ng/mL Plasma, Serum, Tissue Homogenate
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样本量
- 100 μL
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实验时间
- 1 - 4.5 h
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板类型
- Pre-coated
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实验流程
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1. Prepare all reagents, samples and standards
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 µL Stop Solution. Read at 450nm immediately. -
限制
- 仅限研究用
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注意事项
- The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
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注意事项
- The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance. 2. The kit should not be used beyond the expiration date on the kit label. 3. Do not mix or substitute reagents with those from other lots or sources. 4. If samples generate values higher than the highest standard, further dilute the samples with the Sample Diluent and repeat the assay. Any variation in standard diluent, operator, pipetting technique, washing technique,incubation time or temperature, and kit age can cause variation in binding.
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储存条件
- 4 °C
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有效期
- 12 months
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- FSH (Follicle-Stimulating Hormone (FSH))
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别名
- FSH
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物质类
- Hormone
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途径
- Peptide Hormone Metabolism, Chromatin Binding
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